Dalgaard J Z, Silva G H, Belfort M, Van Roey P
Wadsworth Center, New York State Department of Health, PO Box 509, Albany, NY 12201, USA.
Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1435-6. doi: 10.1107/s0907444998006222.
Two forms of the archaeal intron-encoded site-specific endonuclease I-DmoI, namely I-DmoIc and I-DmoIl, have been purified and crystallized. Crystals of I-DmoIc are rod-shaped and diffract to 3.0 A resolution, but further analysis was hampered by twinning. Crystals of I-DmoIl, which is a six-amino-acid C-terminal truncation of I-DmoIc, are plate shaped and belong to space group C2 with cell parameters a = 93.72, b = 37.03, c = 55.56 A, beta = 113.4 degrees, with one molecule per asymmetric unit (Vm = 2.01 A3 Da-1). The crystals diffract to at least 2.3 A resolution. A complete native data set has been measured and structure determination is on-going.
已纯化并结晶出古细菌内含子编码的位点特异性内切核酸酶I-DmoI的两种形式，即I-DmoIc和I-DmoIl。I-DmoIc的晶体呈棒状，衍射分辨率为3.0埃，但孪生现象阻碍了进一步分析。I-DmoIl是I-DmoIc的C末端截短六个氨基酸的形式，其晶体呈板状，属于空间群C2，晶胞参数为a = 93.72、b = 37.03、c = 55.56埃，β = 113.4°，每个不对称单元有一个分子（Vm = 2.01埃³ Da⁻¹）。这些晶体的衍射分辨率至少为2.3埃。已测量了完整的天然数据集，结构测定正在进行中。
