Golubev A M, Lee W, Marangoni S, Novello J C, Oliveira B, Toyama M H, Polikarpov I
Laboratório Nacional de Luz Síncrotron, Caixa Postal 6192, CEP 13083-970, Campinas, São Paulo, Brazil.
Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1440-1. doi: 10.1107/s0907444998007197.
Scorpion neurotoxin Ts-gamma was isolated from Tityus serrulatus venom and purified to apparent homogeneity by ion-exchange HPLC. Crystals of the toxin were grown using polyethylene glycol 6000 as precipitant and were found to belong to the monoclinic space group P21 with cell parameters a = 22.20, b = 36.90, c = 31.57 A, beta = 100.85 degrees. The crystals diffract beyond 1.73 A resolution at a synchrotron beamline, being notably stable during X-ray exposure. The structure has been solved by molecular replacement using the very high resolution structure of Sahara scorpion Androctonus australis Hector (PDB code 1AHO) as a search model.
从锯齿蝎毒液中分离出蝎神经毒素Ts-γ，并通过离子交换高效液相色谱法将其纯化至表观均一性。使用聚乙二醇6000作为沉淀剂培养毒素晶体，发现其属于单斜空间群P21，晶胞参数为a = 22.20，b = 36.90，c = 31.57 Å，β = 100.85°。这些晶体在同步加速器光束线上的衍射分辨率超过1.73 Å，在X射线照射期间非常稳定。通过分子置换法，以撒哈拉蝎黄肥尾蝎（PDB代码1AHO）的超高分辨率结构作为搜索模型，解析了该结构。
