Guan R J, Xiang Y, Wang M, Li G P, Wang D C
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1313-5. doi: 10.1107/s0907444901011210. Epub 2001 Aug 23.
Depressant insect toxins are a distinct group of scorpion neurotoxins for which no three-dimensional structures are yet available. A depressant insect toxin named BmK dITAP3 from the scorpion Buthus martensii Karsch (BmK) has been purified and crystallized. Single crystals of dITAP3 grew in the presence of the detergent CYMAL-6 using the hanging-drop vapour-diffusion method with ammonium sulfate as precipitant. A set of diffraction data to 2.6 A resolution has been collected. Preliminary analysis of the diffraction data indicated that the crystal belonged to space group R3, with unit-cell parameters a = b = 73.29, c = 68.90 A, alpha = beta = 90, gamma = 120 degrees. Assuming two molecules in the asymmetric unit, the estimated solvent content is 53.4%.
抑制性昆虫毒素是一类独特的蝎子神经毒素，目前尚无其三维结构。一种来自东亚钳蝎（BmK）的名为BmK dITAP3的抑制性昆虫毒素已被纯化并结晶。dITAP3的单晶在去污剂CYMAL-6存在的情况下，采用悬滴气相扩散法，以硫酸铵作为沉淀剂生长而成。已收集到分辨率为2.6埃的一组衍射数据。对衍射数据的初步分析表明，该晶体属于R3空间群，晶胞参数a = b = 73.29，c = 68.90埃，α = β = 90°，γ = 120°。假设不对称单元中有两个分子，估计溶剂含量为53.4%。
