Tokura T, Ito S, Nishikawa M, Yamane A, Miki H
Department of Ophthalmology, Kansai Medical University, Japan.
Nippon Ganka Gakkai Zasshi. 1999 Feb;103(2):85-91.
We investigated the effect of high cholesterol diet for the aging changes in Bruch's membrane of rats.
After feeding a 4% cholesterol diet for 15 weeks to three young rats 3 months old and four aged rats 23 months old, we observed the morphological changes of Bruch's membrane by electron microscopy, and made a comparison with rats fed an ordinary diet.
In one young rat fed a high-cholesterol diet, the endothelial basement membrane of the choriocapillaris formed multiple folds separated from the plasma membrane of the endothelium and showed lamellar thickening and crack in some areas. The elastic fiber layer in Bruch's membrane disappeared partly and some new microfibrils appeared. In one aged rat fed a high-cholesterol diet, the endothelial basement membrane of the choriocapillaris showed more lamellar thickening with lumps in some parts. Compared with rats fed an ordinary diet, rats fed a high-cholesterol diet showed thickening of the basement membrane and the changes were more severe.
Our data indicated that high-cholesterol diet might promote age-related changes of Bruch's membrane.
我们研究了高胆固醇饮食对大鼠布鲁赫膜衰老变化的影响。
对3只3个月大的年轻大鼠和4只23个月大的老年大鼠喂食4%胆固醇饮食15周后，通过电子显微镜观察布鲁赫膜的形态变化，并与喂食普通饮食的大鼠进行比较。
在一只喂食高胆固醇饮食的年轻大鼠中，脉络膜毛细血管的内皮基底膜形成了与内皮细胞质膜分离的多个褶皱，并且在某些区域出现了层状增厚和裂缝。布鲁赫膜中的弹性纤维层部分消失，出现了一些新的微纤维。在一只喂食高胆固醇饮食的老年大鼠中，脉络膜毛细血管的内皮基底膜在某些部位表现出更多的层状增厚并伴有肿块。与喂食普通饮食的大鼠相比，喂食高胆固醇饮食的大鼠基底膜增厚，且变化更严重。
我们的数据表明，高胆固醇饮食可能会促进布鲁赫膜的年龄相关变化。
