Tokura T, Ito S, Nishikawa M, Yamane A, Miki H
Department of Ophthalmology, Kansai Medical University, Japan.
Nippon Ganka Gakkai Zasshi. 1999 Feb;103(2):77-84.
We investigated rats were worth the experimental animal on the aging changes in Bruch's membrane.
We did an ultramicromorphological study on the aging changes in Bruch's membrane, in male Sprauge-Dawley rats at 1,6,12 and 27 months after their birth.
With the aging, the basement membranes on both sides of Bruch's membrane have become thick. At 6 months of age, the basement membranes of the endothelial cells of the choriocapillaris were found to be multilayered and cracked and pseudopodia of the endothelium were also observed toward the retinal pigment epithelium. At 12 months of age, we found vacuoles in the basal portion of the retinal pigment epithelium, as well as fibrillae and a part of the process of a cell in the outer collagenous zone correspond with the portion of the multilayered and cracked basement membranes of the endothelial cells of the choriocapillaris. At the age of 27 months, the basement membranes were thicker and a basal laminar deposit was seen. Prominent changes such as disappearance of the elastic fibers and increase of the collagenous fibers and fibrillae were also seen in some portions.
Thus, the aging changes in Bruch's membrane of rats were remarkably reinforced in accordance with the age, it were appeared these rats were worth the experimental animal.
我们研究了大鼠作为实验动物在布鲁赫膜老化变化方面的价值。
我们对出生后1、6、12和27个月的雄性斯普拉格-道利大鼠的布鲁赫膜老化变化进行了超微形态学研究。
随着年龄增长，布鲁赫膜两侧的基底膜变厚。在6个月大时，发现脉络膜毛细血管内皮细胞的基底膜多层且破裂，还观察到内皮细胞朝向视网膜色素上皮的伪足。在12个月大时，我们在视网膜色素上皮的基部发现空泡，以及外胶原带中的纤维和一个细胞的部分突起，这与脉络膜毛细血管内皮细胞多层且破裂的基底膜部分相对应。在27个月大时，基底膜更厚，可见基底膜沉积物。在一些部位还可见到弹性纤维消失、胶原纤维和纤维增加等显著变化。
因此，大鼠布鲁赫膜的老化变化随年龄显著增强，表明这些大鼠适合作为实验动物。
