Binkley N, Ellison G, O'Rourke C, Hall D, Johnston G, Kimmel D, Keller E T
Wisconsin Regional Primate Research Center, University of Wisconsin-Madison, USA.
Lab Anim Sci. 1999 Feb;49(1):87-9.
Old World primates are often studied to model human skeletal physiology. An important advantage of monkeys over other animal models (i.e., rodents) is the presence of cortical bone Haversian remodeling. Seventy-five female rhesus monkeys (Macaca mulatta) were subjected to bone biopsy. With monkeys in lateral decubitus position, the tenth rib was surgically exposed and freed from periosteum by use of careful sharp and blunt dissection. The rib section was resected, using bone cutters, and the surgical wound was closed. This procedure was repeated for the contralateral rib at a later time point in 65 monkeys. There was no mortality or appreciable morbidity. The bone specimens were (mean +/- SD) 2.50 +/- 0.25 cm long, with 5.5 +/- 1.0 mm2 total cross-sectional area. They were adequate for histologic, immunohistochemical, and quantitative histomorphometric examinations. Prevalence of pneumothorax was approximately 8.0% for the 140 procedures. This complication was immediately and successfully corrected by insertion of a small thoracic tube, evacuation of pneumothorax, and closure of the incision. This well-tolerated, repeatable procedure yields excellent specimens for performance of cortical bone histologic examination without euthanasia, allowing longitudinal evaluation.
旧世界灵长类动物常被用于模拟人类骨骼生理学。猴子相对于其他动物模型（即啮齿动物）的一个重要优势是存在皮质骨哈弗斯重塑。对75只雌性恒河猴（猕猴）进行了骨活检。将猴子置于侧卧位，通过仔细的锐性和钝性解剖手术暴露第十肋骨并去除骨膜。使用骨剪切除肋骨部分，然后缝合手术伤口。在稍后的时间点，对65只猴子的对侧肋骨重复此操作。没有出现死亡或明显的发病情况。骨标本长度为（均值±标准差）2.50±0.25厘米，总横截面积为5.5±1.0平方毫米。它们足以进行组织学、免疫组织化学和定量组织形态计量学检查。在140次手术中，气胸的发生率约为8.0%。通过插入一根小胸管、排出气胸并关闭切口，立即成功纠正了这一并发症。这种耐受性良好、可重复的操作无需安乐死即可获得用于皮质骨组织学检查的优质标本，从而允许进行纵向评估。
