Bohm R P, Rockar R A, Ratterree M S, Blanchard J L, Harouse J, Gettie A, Cheng-Mayer C
Tulane Regional Primate Research Center, Department of Veterinary Medicine, Covington, LA, USA.
Contemp Top Lab Anim Sci. 2000 Nov;39(6):24-6.
To support an infectious disease study, we developed a surgical procedure to collect serial thymic biopsies in rhesus monkeys (Macaca mulatta). In many instances in which thymic tissue is required from living animals, open surgical approaches (thoracotomies) are used, which result in greater postoperative pain and longer recovery periods than those associated with thoracoscopic procedures. Our intent was to develop a surgical procedure that allowed serial biopsy of the thymus with minimal surgical morbidity. We modified a previously published experimental method of thoracoscopic total thymectomy in the dog to collect thymic biopsies in M. mulatta. Of the 15 animals evaluated, 8 underwent two biopsy procedures separated by a 5- to 6-week interoperative interval. The other seven animals underwent a single biopsy procedure. Thymic tissue was collected successfully during all procedures, with an average surgical time of 15 min. No significant intra- or postoperative complications were noted, and the animals recoveries from the surgical procedures were uneventful. Minimally invasive thoracoscopic surgical techniques can be used successfully to collect thymic tissue from adult and juvenile rhesus monkeys with minimal surgical morbidity.
为支持一项传染病研究，我们开发了一种手术程序，用于在恒河猴（猕猴）身上采集系列胸腺活检样本。在许多需要从活体动物获取胸腺组织的情况下，会采用开放手术方法（开胸术），与胸腔镜手术相比，这种方法会导致术后疼痛更剧烈，恢复期更长。我们的目的是开发一种手术程序，能够以最小的手术发病率进行胸腺系列活检。我们修改了先前发表的犬类胸腔镜全胸腺切除术的实验方法，以在恒河猴身上采集胸腺活检样本。在评估的15只动物中，8只接受了两次活检程序，两次手术间隔为5至6周。另外7只动物接受了单次活检程序。在所有手术过程中均成功采集到胸腺组织，平均手术时间为15分钟。未观察到明显的术中或术后并发症，动物从手术中的恢复过程顺利。微创胸腔镜手术技术可成功用于从成年和幼年恒河猴身上采集胸腺组织，且手术发病率最小。
