Hoy T G, Harrison P M
Br J Haematol. 1976 Aug;33(4):497-504. doi: 10.1111/j.1365-2141.1976.tb03568.x.
The incorporation of ferric iron labelled with 59Fe into rat liver ferritin has been studied in whole animals, into liver homogenate and into purified protein. Uptake of Fe3+ into purified rat liver ferritin followed a pattern similar to that with horse spleen ferritin given Fe2+ and an oxidant. The distributions of iron incorporated as a function of molecular iron content obtained in vivo resembled the in vitro patterns for iron contents above 500 Fe atoms/molecule and times after injection of up to 12 h. At larger intervals a maximum label moved to molecules of highest iron content as the molecules accumulated more iron. The apparently reduced uptake of injected 59Fe into molecules of low iron content might be due either to the chase of cold iron through an existing iron pool or the presence of functionally different ferritins at more than one anatomic site within the cell.
已在整体动物、肝脏匀浆和纯化蛋白中研究了用59Fe标记的三价铁掺入大鼠肝脏铁蛋白的情况。Fe3+掺入纯化的大鼠肝脏铁蛋白的模式，与给马脾铁蛋白提供Fe2+和一种氧化剂时的模式相似。在体内获得的作为分子铁含量函数的掺入铁的分布，对于铁含量高于500个铁原子/分子以及注射后长达12小时的时间而言，类似于体外模式。在更长的时间间隔后，随着分子积累更多的铁，最大标记转移到铁含量最高的分子上。注射的59Fe明显减少地掺入低铁含量分子中，这可能是由于通过现有的铁池追踪冷铁，或者是由于细胞内不止一个解剖部位存在功能不同的铁蛋白。
