铁蛋白对三价铁的结合。
The binding of ferric iron by ferritin.
作者信息
Treffry A, Harrison P M
出版信息
Biochem J. 1979 Sep 1;181(3):709-16. doi: 10.1042/bj1810709.
Abstract
Equilibrium-dialysis experiments with 59Fe-labelled Fe(III) chelate solutions show that ferritin is capable of binding a limited number of Fe(III) atoms. Some of this Fe(III) is readily removed, but up to about 200 Fe(III) atoms/molecule remain bound after extensive washing. Some exchange of labelled Fe(III) with endogenous unlabelled ferritin Fe occurs during prolonged dialysis against 59Fe(III)-citrate, but there is a net binding of Fe(III). Bound Fe(III) resembles endogenous Fe(III) in several respects. It appears to be attached to the micelle and not to the protein component of ferritin. Although the physiological mechanism of Fe incorporation into ferritin is unknown, our experiments suggest the possibility that some iron finds its way into ferritin as Fe(III) chelate.
摘要
用59Fe标记的Fe(III)螯合物溶液进行的平衡透析实验表明，铁蛋白能够结合有限数量的Fe(III)原子。其中一些Fe(III)很容易被去除，但经过大量洗涤后，每个分子仍有约200个Fe(III)原子保持结合状态。在与59Fe(III)-柠檬酸盐进行长时间透析期间，标记的Fe(III)与内源性未标记的铁蛋白铁会发生一些交换，但存在Fe(III)的净结合。结合的Fe(III)在几个方面类似于内源性Fe(III)。它似乎附着在微胶粒上，而不是铁蛋白的蛋白质成分上。尽管铁掺入铁蛋白的生理机制尚不清楚，但我们的实验表明，一些铁可能以Fe(III)螯合物的形式进入铁蛋白。
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