Treffry A, Lee P J, Harrison P M
Biochim Biophys Acta. 1984 Feb 28;785(1-2):22-9. doi: 10.1016/0167-4838(84)90229-2.
Rat-liver and horse-spleen isoferritins were obtained by preparative isoelectric focussing and several of these were fractionated further by sucrose density gradient centrifugation. H- and L-subunit compositions were also measured. Isoferritins were found to have neither a fixed iron content nor a unique subunit composition. In both species within a single isoferritin a small increase in the percentage of H subunit paralleled increasing iron content. Although in horse-spleen ferritin a similar correlation was found over the isoferritin profile as a whole, this was not generally true of rat-liver isoferritins, since iron distributions varied with the iron status of the animals. Rates of iron incorporation into isoapoferritins were measured in vitro and the distribution of 59Fe among rat liver isoferritins was measured at various times after injection of 59Fe. The data do not support the proposal that, in rat liver, L-rich isoferritins are the preferred iron-storage form.
通过制备性等电聚焦获得大鼠肝脏和马脾脏异铁蛋白，其中一些再通过蔗糖密度梯度离心进一步分级分离。还测定了H亚基和L亚基的组成。发现异铁蛋白既没有固定的铁含量，也没有独特的亚基组成。在单个异铁蛋白中，两种动物的H亚基百分比的小幅增加与铁含量的增加平行。尽管在马脾脏铁蛋白中，在整个异铁蛋白谱中发现了类似的相关性，但大鼠肝脏异铁蛋白通常并非如此，因为铁分布随动物的铁状态而变化。在体外测量了铁掺入脱铁异铁蛋白的速率，并在注射59Fe后的不同时间测量了59Fe在大鼠肝脏异铁蛋白中的分布。这些数据不支持在大鼠肝脏中富含L的异铁蛋白是首选铁储存形式的提议。
