Kapicioğlu Z, Kalyoncu I N, Deger O, Can G
Karadeniz Technical University, School of Medicine, Department of Ophthalmology, Pharmacology, Biochemistry and Public Health, Trabzon, Turkey.
Int Ophthalmol. 1998;22(1):43-5. doi: 10.1023/a:1006171323980.
In this study we investigated the effect of a somatostatin analogue, octreotide, (SMS 201-995) on tear secretion in rats.
The animals were anaesthetized intraperitoneally (ip) with urethan (1.2 g/kg) The drugs were injected subcutaneously (sc.). Tear samples were collected by folding a 5 mm section of the Schirmer strip over the lower lid margin to absorb tear fluid from the lower conjunctival sac for 5 mm. Forty animals were divided into four groups (n = 10). Group I (control) received 1 ml of saline, group 2 received 100 microg/kg of SMS 201-995, group 3 received 50 microg/kg of acetylcholine (Ach), group 4 received 100 microg/kg of SMS 201-995 and 30 min later 50 microg/kg of Ach. At t = 0 the local anesthetic proparacain HCI was instilled onto the ocular surface to minimize reflex secretion. Baseline secretion was measured before administering the various treatments.
While Ach alone significantly increased tear secretion, SMS 201-995 reduced it compared to saline (control) (p<0.0001, p<0.001, respectively). SMS 201-995 combined with Ach decreased tear secretion compared to Ach alone (p<0.03).
SMS 201-995 significantly inhibits Ach stimulated tear secretion in rats.
在本研究中，我们调查了生长抑素类似物奥曲肽（SMS 201 - 995）对大鼠泪液分泌的影响。
动物通过腹腔注射（ip）氨基甲酸乙酯（1.2 g/kg）麻醉。药物皮下注射（sc.）。通过将Schirmer试纸的5毫米部分折叠在下睑缘上来收集泪液样本，以从下结膜囊吸收泪液5毫米。40只动物分为四组（n = 10）。第一组（对照组）接受1毫升生理盐水，第二组接受100微克/千克的SMS 201 - 995，第三组接受50微克/千克的乙酰胆碱（Ach），第四组接受100微克/千克的SMS 201 - 995，30分钟后接受50微克/千克的Ach。在t = 0时，将局部麻醉剂盐酸丙美卡因滴入眼表面以尽量减少反射性分泌。在给予各种治疗之前测量基础分泌。
单独使用Ach时泪液分泌显著增加，而与生理盐水（对照组）相比，SMS 201 - 995降低了泪液分泌（分别为p<0.0001，p<0.001）。与单独使用Ach相比，SMS 201 - 995与Ach联合使用时泪液分泌减少（p<0.03）。
SMS 201 - 995显著抑制Ach刺激的大鼠泪液分泌。
