Lehmann M, Groh A, Rödel J, Nindl I, Straube E
Department of Medical Microbiology, Friedrich Schiller University of Jena, Germany.
J Infect. 1999 Jan;38(1):12-7. doi: 10.1016/s0163-4453(99)90021-x.
The correlation between human papillomavirus (HPV) and Chlamydia, trachomatis infections was evaluated in 144 patients with normal cytology or with atypical squamous cells of undetermined significance (ASCUS).
Cervical samples were analysed using polymerase chain reaction (PCR) and non-radioactive Southern blot analysis. Specificity and sensitivity of two C. trachomatis PCR systems: major outer membrane protein (MOMP)-PCR and plasmid-PCR were determined. Southern blot hybridization of the PCR amplicons was done using 5' and 3' biotinylated oligonucleotide probes.
All cervical samples were tested by the plasmid-PCR due to a 10 times higher sensitivity compared to the MOMP-PCR. To determine the specificity of our C. trachomatis primer sets different bacteria and viruses which can cause urogenital infections were analysed. Comparison of the probes revealed an increased sensitivity of the 5' and 3' double-biotinylated probe vs. the 5' biotinylated probe. The infection rate of C. trachomatis in cervical samples of HPV-positive patients was 10.3% (three out of 29) vs. 1.7% (two out of 115; P< or =0.05) in HPV-negative patients. In patients HPV-X (unsequenced HPV-types) positive the rate was 14.3% (one out of seven) vs. 2.9% (four out of 137: P = 0.2) in HPV-X negative patients. In high risk (HR) HPV-positive cervical samples the infection rate was 9.1% (two out of 22) vs. 2.5% (three out of 122; P = 0.14) in HR HPV-negative samples. Chlamydia trachomatis frequency of patients with cytological changes (ASCUS) was 27.3% (three out of 11) vs. 1.5% (two out of 1 33) in patients with normal cytology (P = 0.003). The highest prevalence rate of C. trachomatis-positive cervical samples (50%; one out of two) was found in HR HPV-positive patients with cytological changes (ASCUS) vs. 5% (one out of 20) in HR HPV-positive patients with normal cytology (P = 0.17). Patients negative for HPV and positive for ASCIIS have a C. trachomatis rate of 22.2% (two out of nine) vs. HPV-negative patients with normal cytology (none out of 106; P = 0.006) and vs. HR HPV-negative patients with normal cytology (0.9%; one out of 113; P = 0.014).
There appears to be a correlation between cervical HPV and cervical C. trachomatis infections. The prevalence rate of C. trachomatis was significantly higher in patients with abnormal cytology (ASCUS) vs. normal cytology.
在144例细胞学正常或意义不明确的非典型鳞状细胞（ASCUS）患者中评估人乳头瘤病毒（HPV）与沙眼衣原体感染之间的相关性。
采用聚合酶链反应（PCR）和非放射性Southern印迹分析对宫颈样本进行检测。测定了两种沙眼衣原体PCR系统（主要外膜蛋白（MOMP）-PCR和质粒-PCR）的特异性和敏感性。使用5'和3'生物素化寡核苷酸探针进行PCR扩增产物的Southern印迹杂交。
由于质粒-PCR的敏感性比MOMP-PCR高10倍，所有宫颈样本均采用质粒-PCR检测。为确定我们的沙眼衣原体引物组的特异性，分析了可能导致泌尿生殖道感染的不同细菌和病毒。探针比较显示，5'和3'双生物素化探针比5'生物素化探针的敏感性更高。HPV阳性患者宫颈样本中沙眼衣原体感染率为10.3%（29例中的3例），而HPV阴性患者为1.7%（115例中的2例；P≤0.05）。HPV-X（未测序HPV型别）阳性患者中该率为14.3%（7例中的1例），而HPV-X阴性患者为2.9%（137例中的4例；P = 0.2）。高危（HR）HPV阳性宫颈样本中感染率为9.1%（22例中的2例），而HR HPV阴性样本为2.5%（122例中的3例；P = 0.14）。细胞学改变（ASCUS）患者中沙眼衣原体感染率为27.3%（11例中的3例），而细胞学正常患者为1.5%（133例中的2例；P = 0.003）。沙眼衣原体阳性宫颈样本患病率最高（50%；2例中的1例）的是HR HPV阳性且有细胞学改变（ASCUS）的患者，而HR HPV阳性且细胞学正常的患者中为5%（20例中的1例；P = 0.17）。HPV阴性且ASCUS阳性的患者中沙眼衣原体感染率为22.2%（9例中的2例），而HPV阴性且细胞学正常的患者（106例中无）（P = 0.006）以及HR HPV阴性且细胞学正常的患者（0.9%；113例中的1例；P = 0.014）。
宫颈HPV与宫颈沙眼衣原体感染之间似乎存在相关性。细胞学异常（ASCUS）患者中沙眼衣原体患病率显著高于细胞学正常患者。
