镁缺乏时大鼠组织中碱性磷酸酶（EC 3.1.3.1）和无机焦磷酸酶（EC 3.6.1.1）活性的变化。控制喂养模式的重要性。

Changes in the alkaline phosphatase (EC 3.1.3.1) and inorganic pyrophosphatase (EC 3.6.1.1) activities of rat tissues during magnesium deficiency. The importance of controlling feeding pattern.

Loveless B W, Heaton F W

Br J Nutr. 1976 Nov;36(3):487-95. doi: 10.1079/bjn19760103.

Abstract

The adoption of a meal-eating pattern of feeding by rats altered the alkaline phosphatase (EC 3.1.3.1) activity in serum and liver. It was therefore necessary to regulate the feeding pattern of both magnesium-deficient rats and control animals receiving a Mg-adequate diet in order to study the effect of the deficiency. 2. Mg deficiency decreased the activities of alkaline phosphatase and inorganic pyrophosphatase (EC 3.6.1.1) in serum, kidney and tibia, but increased them in spleen. 3. Addition of a standard concentration of exogenous Mg to tissue extracts usually increased the activity of corresponding enzymes from Mg-deficient and control rats by the same proportion, indicating that the main effect of the deficiency was on the amount of enzyme present rather than on the efficiency of its operation. 4. Certain quantitative differences in the response to exogenous Mg and the activity ratio, alkaline phosphatase:inorganic pyrophosphatase were found between tissues from Mg-deficient and control rats. The significance of these are discussed in relation to the association of the two enzymic activities with the same protein molecule, and the possible occurrence of isoenzymes.

摘要

采用进食模式喂养大鼠会改变血清和肝脏中碱性磷酸酶（EC 3.1.3.1）的活性。因此，为了研究镁缺乏的影响，有必要对缺镁大鼠和摄入镁充足饮食的对照动物的喂养模式进行调节。2. 镁缺乏会降低血清、肾脏和胫骨中碱性磷酸酶和无机焦磷酸酶（EC 3.6.1.1）的活性，但会增加脾脏中这些酶的活性。3. 向组织提取物中添加标准浓度的外源性镁通常会使缺镁大鼠和对照大鼠相应酶的活性按相同比例增加，这表明缺乏的主要影响在于酶的含量而非其运作效率。4. 在缺镁大鼠和对照大鼠的组织之间，发现了对外源性镁的反应以及碱性磷酸酶与无机焦磷酸酶活性比的某些定量差异。结合这两种酶活性与同一蛋白质分子的关联以及同工酶可能的存在情况，对这些差异的意义进行了讨论。