镁缺乏对大鼠组织中碱性磷酸酶（EC 3.1.3.1）同工酶的差异影响。

The differential effect of magnesium deficiency on isoenzymes of alkaline phosphatase (EC 3.1.3.1) in rat tissues.

Nehlawi M F, Heaton F W

Br J Nutr. 1979 Jul;42(1):105-11. doi: 10.1079/bjn19790094.

Abstract

Alkaline phosphatase (EC 3.1.3.1) from many rat tissues was separated into two or three bands by electrophoresis on polyacrylamide gel. Ten of the bands had different electrophoretic mobilities, but some were present in more than one tissue. 2. Bands from the corresponding tissues of magnesium-deficient and control rats were qualitatively similar, but there were quantitative differences in the distribution of enzyme activity between them. 3. Mg deficiency had differential effects on the absolute activity of individual bands, but two main types of response were observed. There was an increase in the activity of the first bands from liver and kidney, the second band from femur and both bands from spleen, whereas the first band from femur, the first and second bands from intestinal mucosa and the second bands of serum, liver and kidney all decreased in activity during the deficiency. 4. The change in total alkaline phosphatase activity of a tissue during Mg deficiency depended on the ratio between the enzyme components within it.

摘要

通过在聚丙烯酰胺凝胶上进行电泳，来自许多大鼠组织的碱性磷酸酶（EC 3.1.3.1）被分离成两条或三条带。其中十条带具有不同的电泳迁移率，但有些带存在于不止一种组织中。2. 缺镁大鼠和对照大鼠相应组织的条带在质量上相似，但它们之间酶活性的分布存在数量差异。3. 缺镁对各个条带的绝对活性有不同影响，但观察到两种主要的反应类型。肝脏和肾脏的第一条带、股骨的第二条带以及脾脏的两条带的活性增加，而股骨的第一条带、肠黏膜的第一条和第二条带以及血清、肝脏和肾脏的第二条带在缺镁期间活性均降低。4. 组织在缺镁期间总碱性磷酸酶活性的变化取决于其内部酶成分之间的比例。