Odaka C, Toyoda E, Nemoto K
Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Tokyo, Japan.
J Antibiot (Tokyo). 1999 Jan;52(1):45-51. doi: 10.7164/antibiotics.52.45.
Deoxyspergualin (DSG) has both antitumor and immunosuppressive activities. We explored the mechanism of DSG activities using an aqueous soluble analogue, methyldeoxyspergualin (MeDSG) for in vitro culture studies. It is known that DSG has inhibitory effects on cell proliferation, and we also observed that MeDSG inhibited [3H]-thymidine incorporation by rapidly dividing murine T cell hybridomas. However, when tetrazolium (MTT) colorimetric assay was adopted to evaluate its inhibitory effects on cell proliferation, MeDSG induced an enhanced MTT reduction. When we examined whether these results were applicable to the actively dividing cells of other origins than T cells, similar effects were seen with Raji cells, J774.1 cells and NIH3T3 cells. N-30, another analogue which was capable of suppressing anti-SRBC antibody production in vivo, also induced inhibition of cell growth and an enhanced MTT reduction. In contrast, the analogue which failed to prevent the antibody production, neither enhanced MTT reduction nor inhibited cell proliferation. Our results demonstrated that the ability to generate MTT formazan in dividing cells is a common property among, DSG analogue with the immunosuppressive and antiproliferative activities.
去氧精胍菌素（DSG）具有抗肿瘤和免疫抑制活性。我们使用水溶性类似物甲基去氧精胍菌素（MeDSG）进行体外培养研究，以探索DSG活性的机制。已知DSG对细胞增殖有抑制作用，并且我们还观察到MeDSG抑制快速分裂的小鼠T细胞杂交瘤的[3H] - 胸腺嘧啶核苷掺入。然而，当采用四氮唑（MTT）比色法评估其对细胞增殖的抑制作用时，MeDSG导致MTT还原增强。当我们检查这些结果是否适用于除T细胞以外的其他来源的活跃分裂细胞时，在Raji细胞、J774.1细胞和NIH3T3细胞中也观察到了类似的效果。N - 30是另一种能够在体内抑制抗SRBC抗体产生的类似物，它也诱导细胞生长抑制和MTT还原增强。相反，未能阻止抗体产生的类似物既没有增强MTT还原，也没有抑制细胞增殖。我们的结果表明，在具有免疫抑制和抗增殖活性的DSG类似物中，在分裂细胞中产生MTT甲臜的能力是一种共同特性。
