Veenstra T D
Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
Biochem Biophys Res Commun. 1999 Apr 2;257(1):1-5. doi: 10.1006/bbrc.1998.0103.
With the emergence of electrospray ionization mass spectrometry (ESI-MS), mass spectrometry is no longer restricted to the study of small, stable molecules, but has become a viable technique to study large biomolecules as well as noncovalent biomolecular complexes. ESI-MS has been used to study noncovalent interactions involving proteins with metals, ligands, peptides, oligonucleotides, and other proteins. An area where ESI-MS holds significant promise is in the study of protein/DNA interactions. The most common technique employed to study protein/DNA interactions is the electrophoretic gel mobility shift assay (EMSA). Although this technique has and will continue to provide excellent results, ESI-MS has shown the ability to provide detailed results not easily obtainable by EMSA. In this review I will discuss some of the protein/DNA noncovalent interactions that have been measured using ESI-MS, and contrast the results obtained by ESI-MS to those obtained by EMSA.
随着电喷雾电离质谱（ESI-MS）的出现，质谱不再局限于对小的稳定分子的研究，而是已成为研究大生物分子以及非共价生物分子复合物的可行技术。ESI-MS已被用于研究涉及蛋白质与金属、配体、肽、寡核苷酸及其他蛋白质的非共价相互作用。ESI-MS具有重大前景的一个领域是蛋白质/DNA相互作用的研究。用于研究蛋白质/DNA相互作用的最常用技术是电泳凝胶迁移率变动分析（EMSA）。尽管该技术已经并将继续提供出色的结果，但ESI-MS已显示出能够提供一些EMSA不易获得的详细结果。在本综述中，我将讨论一些使用ESI-MS测量的蛋白质/DNA非共价相互作用，并将ESI-MS获得的结果与EMSA获得的结果进行对比。
