肥胖状态下脂肪特异性蛋白脂联素出现反常减少。

Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity.

作者信息

Arita Y, Kihara S, Ouchi N, Takahashi M, Maeda K, Miyagawa J, Hotta K, Shimomura I, Nakamura T, Miyaoka K, Kuriyama H, Nishida M, Yamashita S, Okubo K, Matsubara K, Muraguchi M, Ohmoto Y, Funahashi T, Matsuzawa Y

机构信息

Graduate School of Medicine, Institute for Molecular and Cellular Biology, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.

出版信息

Biochem Biophys Res Commun. 1999 Apr 2;257(1):79-83. doi: 10.1006/bbrc.1999.0255.

DOI:10.1006/bbrc.1999.0255

PMID:10092513

Abstract

We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K., et al., Biochem. Biophys. Res. Commun. 221, 286-289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans.

摘要

我们分离出了人类脂肪组织特异性且最为丰富的基因转录本apM1（前田健等人，《生物化学与生物物理研究通讯》，第221卷，第286 - 289页，1996年）。apM1基因产物是一种可溶性基质蛋白，我们将其命名为脂联素。为了定量血浆脂联素浓度，我们制备了针对人类脂联素的单克隆抗体和多克隆抗体，并开发了一种酶联免疫吸附测定（ELISA）系统。脂联素大量存在于健康志愿者的血浆中，浓度范围为1.9至17.0毫克/毫升。肥胖受试者血浆中的脂联素浓度显著低于非肥胖受试者，尽管脂联素仅由脂肪组织分泌。本研究中开发的ELISA系统将有助于阐明脂联素在人类生理和病理生理过程中的作用。