Shimizu S, Nomura K, Ujihara M, Demura H
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical University School of Medicine, Tokyo, 162-8666, Japan.
Biochem Biophys Res Commun. 1999 Apr 2;257(1):193-8. doi: 10.1006/bbrc.1999.0433.
The HSP70-1 gene, reportedly a single exon, encodes a major stress-inducible 72-kDa heat shock protein (HSP70). We recently demonstrated that patients with major depression had a 162-base pair (bp)-deletion in the 5'-flanking sequence of HSP70-1 mRNA in their peripheral blood mononuclear cells. Present study has been conducted to clarify how this short mRNA is generated, and demonstrated that a novel 385 bp sequence is located 1.1 kb upstream from the HSP70-1 gene of normal subjects. Except for the 162 bp deletion, it is compatible with part of the 5'-flanking sequence of the HSP70-1 gene, and contains another exon of 358 bp (exon 1) that may be connected to the 3'-terminus (exon 3) of the conventional HSP70-1 gene. Alternative transcription of exons 1 and 3 may cause the short mRNA. It is concluded that HSP70-1 gene is constituted of three exons and may cause alternative splicing.
据报道，HSP70-1基因只有一个外显子，编码一种主要的应激诱导型72 kDa热休克蛋白（HSP70）。我们最近发现，重度抑郁症患者外周血单核细胞中HSP70-1 mRNA的5'侧翼序列存在162个碱基对（bp）的缺失。本研究旨在阐明这种短mRNA是如何产生的，并证明在正常受试者的HSP70-1基因上游1.1 kb处存在一个新的385 bp序列。除了162 bp的缺失外，它与HSP70-1基因5'侧翼序列的一部分相符，并包含另一个358 bp的外显子（外显子1），该外显子可能与传统HSP70-1基因的3'末端（外显子3）相连。外显子1和3的选择性转录可能导致短mRNA的产生。结论是，HSP70-1基因由三个外显子组成，可能会导致选择性剪接。
