Shimizu S, Nomura K, Ujihara M, Sakamoto K, Shibata H, Suzuki T, Demura H
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College, Japan.
Biochem Biophys Res Commun. 1996 Feb 27;219(3):745-52. doi: 10.1006/bbrc.1996.0305.
Stress-inducible 72-kDa heat shock proteins (HSP70) were encoded on genes in multiple chromosomes. The expression of mRNA transcribed from the gene (HSP70-1) on chromosome 6 was studied using reverse transcript polymerase chain reaction in peripheral blood mononuclear cells of patients with different diseases. The deletion of 29 bp occurred in 5' noncoding and subsequent 133 bp in coding sequences of HSP70 mRNA in patients with major depression (n = 18), while normal subjects (n = 10) and patients with schizophrenia (n = 5), essential hypertension (n = 3), rheumatoid arthritis (n = 7), and Graves' disease (n = 3) had normal mRNA. No such deletion occurred in genomic DNA and no protein was translated from deleted mRNA. The allel-specific abnormal transcript of the HSP70 gene on chromosome 6 thus may underlie the altered stress and/or immune response in major depression.
应激诱导型72-kDa热休克蛋白（HSP70）由多条染色体上的基因编码。利用逆转录聚合酶链反应，对不同疾病患者外周血单个核细胞中6号染色体上基因（HSP70-1）转录的mRNA表达进行了研究。在重度抑郁症患者（n = 18）中，HSP70 mRNA的5'非编码区出现29 bp缺失，随后编码序列中出现133 bp缺失，而正常受试者（n = 10）、精神分裂症患者（n = 5）、原发性高血压患者（n = 3）、类风湿关节炎患者（n = 7）和格雷夫斯病患者（n = 3）的mRNA正常。基因组DNA未发生此类缺失，缺失的mRNA也未翻译出蛋白质。因此，6号染色体上HSP70基因的等位基因特异性异常转录本可能是重度抑郁症患者应激和/或免疫反应改变的基础。
