Ito K, Ishii N, Miyashita A, Tominaga K, Kuriyama H, Maruyama H, Shirai M, Naito M, Arakawa M, Kuwano R
Research Laboratory for Molecular Genetics, Niigata University, Niigata, 951-8510, Japan.
Biochem Biophys Res Commun. 1999 Apr 2;257(1):206-13. doi: 10.1006/bbrc.1999.0430.
A novel gene was trapped in mouse embryonic stem cells with a promoterless gene trap vector. Fused transcripts were isolated from the embryos by rapid amplification of cDNA ends, which were used for full-length cDNA cloning. The protein predicted from the cDNA consisting of 7143 nucleotides comprises 1184 amino acids, which was confirmed by in vitro transcription/translation assaying. An antibody against the synthesized peptide reacted with an approximate 130-kDa protein on SDS-PAGE. A search of available databases revealed that this protein is a novel protein composed of 17 ankyrin repeats hooked to a zinc finger motif, which we named Ankhzn. Ankhzn was observed on the endosomal membrane on immunoelectron microscopic analysis. Ankhzn belongs to a new subgroup of double zinc finger proteins which may be involved in vesicle or protein transport. Ankhzn mRNA and its protein were expressed ubiquitously from embryonic day 10.5 to adulthood.
利用无启动子基因捕获载体在小鼠胚胎干细胞中捕获了一个新基因。通过cDNA末端快速扩增从胚胎中分离出融合转录本，用于全长cDNA克隆。由7143个核苷酸组成的cDNA预测的蛋白质包含1184个氨基酸，这通过体外转录/翻译分析得到证实。针对合成肽的抗体在SDS-PAGE上与一种约130 kDa的蛋白质发生反应。对现有数据库的搜索显示，该蛋白质是一种由17个锚蛋白重复序列连接到一个锌指基序组成的新蛋白质，我们将其命名为Ankhzn。免疫电子显微镜分析观察到Ankhzn在内体膜上。Ankhzn属于双锌指蛋白的一个新亚组，可能参与囊泡或蛋白质运输。从胚胎第10.5天到成年期，Ankhzn mRNA及其蛋白质在全身广泛表达。
