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[应用间接免疫荧光法鉴定多种猪支原体]

[Identification of various porcine mycoplasmas using epi-immunofluorescence].

作者信息

Zizková P, Gois M, Kuksa F

出版信息

Vet Med (Praha). 1978 Sep;23(9):521-30.

PMID:100927
Abstract

The responses of the field strains and type strains of Mycoplasma hyorhinis are compared in epi-immunofluorescence, in growth-inhibition tests, and growth-precipitation tests. The type strains, together with the field strains, could be definitely identified by direct and indirect epi-immunofluorescence. In indirect epi-immunofluorescence, all rabbit antiserums against the strains of Mycoplasma hyorhinis tested by us always gave positive cross reactions with all the tested strains of Mycoplasma hyorhinis. On the other hand, in the growth-inhibition cross test and growth precipitation cross test, the type strains of Mycoplasma hyorhinis always reacted positively only with the homologous antiserums, whereas the heterologous reactions were mostly negative or dubious. The clearly positive epi-immunofluorescent reactions with serums against any strain of Mycoplasma hyorhinis suggest that this method is more suitable for the identification of Myocplasma species than the other two tests studied.

摘要

比较了猪鼻支原体的田间菌株和标准菌株在表位免疫荧光、生长抑制试验及生长沉淀试验中的反应。标准菌株与田间菌株可通过直接和间接表位免疫荧光明确鉴定。在间接表位免疫荧光中,我们测试的所有抗猪鼻支原体菌株的兔抗血清与所有测试的猪鼻支原体菌株均产生阳性交叉反应。另一方面,在生长抑制交叉试验和生长沉淀交叉试验中,猪鼻支原体标准菌株总是仅与同源抗血清产生阳性反应,而异源反应大多为阴性或可疑。与抗任何猪鼻支原体菌株血清的明显阳性表位免疫荧光反应表明,该方法比所研究的其他两种试验更适合于支原体种类的鉴定。

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