Krogsgaard-Jensen A
Appl Microbiol. 1972 Mar;23(3):553-8. doi: 10.1128/am.23.3.553-558.1972.
A serological test is described which is designated the growth precipitation test and is based on the release of diffusible compounds from mycoplasma strains. During growth of mycoplasmas on solid substrates, precipitation lines are formed with antibodies from homologous antisera independent of heat-labile serum factors. The application of the test as a serodiagnostic tool was investigated by performing a complete serological cross-test with 10 mycoplasma strains. The test was compared with the growth inhibition test, the complement fixation test, and the indirect hemagglutination test. It is demonstrated that the growth precipitation test has a good degree of specificity. The species and subspecies used here showed cross-reactions between serologically closely related strains, but these heterologous reactions occurred only after longer incubation than the homologous reactions.
本文描述了一种血清学检测方法，称为生长沉淀试验，该试验基于支原体菌株释放的可扩散化合物。在支原体在固体培养基上生长期间，与来自同源抗血清的抗体形成沉淀线，而与热不稳定血清因子无关。通过对10株支原体进行完整的血清学交叉试验，研究了该试验作为血清诊断工具的应用。将该试验与生长抑制试验、补体结合试验和间接血凝试验进行了比较。结果表明，生长沉淀试验具有较高的特异性。这里使用的种和亚种在血清学密切相关的菌株之间显示出交叉反应，但这些异源反应仅在比同源反应更长的孵育时间后才出现。
