Bouchard D, Keleher W, Opitz H M, Blake S, Edwards K C, Nicholson B L
Micro Technologies, Inc., Richmond, Maine 04357, USA.
Dis Aquat Organ. 1999 Jan 29;35(2):131-7. doi: 10.3354/dao035131.
Infectious salmon anemia virus (ISAV) was isolated at a marine grow-out site in New Brunswick, Canada, from Atlantic salmon Salmo salar which experienced mortalities due to hemorrhagic kidney syndrome (HKS). Of 20 fish sampled in this study, 14 showed histologically various degrees of interstitial hemorrhaging, tubular epithelial degeneration and necrosis, and tubular casts in the posterior kidney, typical of HKS. Posterior kidney and spleen homogenates produced a cytopathic effect on chinook salmon embryo (CHSE-214) cells 10 to 14 d after inoculation. Pleomorphic virus particles in the size range 80 to 120 nm were seen by electron microscopy. The virus was confirmed as ISAV using reverse transcriptase-polymerase chain reaction (RT-PCR). This is a systematic diagnostic study of the isolation of ISAV on the North American continent and the first description of the growth of ISAV on the CHSE-214 cell line.
传染性鲑鱼贫血病毒（ISAV）是在加拿大新不伦瑞克省一个海水养殖场所，从患有出血性肾病综合征（HKS）而死亡的大西洋鲑鱼（Salmo salar）中分离出来的。在本研究中采集的20条鱼中，有14条在组织学上显示出后肾有不同程度的间质出血、肾小管上皮变性和坏死以及肾小管管型，这是HKS的典型症状。接种后10至14天，后肾和脾脏匀浆对奇努克鲑鱼胚胎（CHSE-214）细胞产生了细胞病变效应。通过电子显微镜观察到大小在80至120纳米范围内的多形病毒颗粒。使用逆转录聚合酶链反应（RT-PCR）确认该病毒为ISAV。这是北美大陆上关于ISAV分离的一项系统性诊断研究，也是首次描述ISAV在CHSE-214细胞系上的生长情况。
