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吉尔维氏纤维弧菌的纤维素酶诱导及以纤维素作为首选生长底物的应用。

Cellulase induction and the use of cellulose as a preferred growth substrate by Cellvibrio gilvus.

作者信息

Breuil C, Kushner D J

出版信息

Can J Microbiol. 1976 Dec;22(12):1776-81. doi: 10.1139/m76-264.

Abstract

Cellvibrio gilvus produced cellulase when grown in the presence of cellulose or carboxymethyl cellulose (CMC) but not when grown in the presence of glucose or cellobiose. This was so whether or not these compounds were the sole carbon sources present. Repeated addition of small amounts of glucose prevented cellulase formation in the presence of cellulose. It is concluded that cellulose and CMC induce cellulase formation and glucose and cellobiose repressit. Cellulose stimulated growth when added to organic media. Physical contact between cells and cellulose fibres was necessary for both cellulase induction and growth stimulation. If cellulase induction was prevented by glucose, cellulose did not stimulate growth.

摘要

吉尔维氏纤维弧菌在以纤维素或羧甲基纤维素(CMC)为碳源生长时会产生纤维素酶,而在以葡萄糖或纤维二糖为碳源生长时则不会产生。无论这些化合物是否为唯一存在的碳源,情况都是如此。在纤维素存在的情况下,反复添加少量葡萄糖可阻止纤维素酶的形成。可以得出结论,纤维素和CMC诱导纤维素酶的形成,而葡萄糖和纤维二糖则抑制其形成。当添加到有机培养基中时,纤维素会刺激生长。细胞与纤维素纤维之间的物理接触对于纤维素酶的诱导和生长刺激都是必需的。如果葡萄糖阻止了纤维素酶的诱导,那么纤维素就不会刺激生长。

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