Saddler J N, Khan A W, Martin S M
Microbios. 1980;28(112):97-106.
A mesophilic anaerobe, Acetivibrio cellulolyticus, isolated from a cellulose-enrichment culture, degraded cellulose by the secretion of cellulolytic enzymes into the culture medium. Both exo- and endoglucanase activities were detected and shown to be regulated by induction and catabolite repression. Endoglucanase synthesis was induced by cellulose, cellobiose and salicin. Synthesis induced during growth on cellobiose was inhibited, although not completely repressed by glucose. Exoglucanase activity was enhanced when cells were grown on insoluble or soluble poly beta-glucoside polymers. Activity was progressively inhibited by supplementing microcrystalline substrates with increasing amounts of cellobiose.
从纤维素富集培养物中分离出的嗜温厌氧菌纤维分解醋弧菌，通过向培养基中分泌纤维素分解酶来降解纤维素。检测到外切葡聚糖酶和内切葡聚糖酶的活性，并表明它们受诱导和分解代谢物阻遏的调节。内切葡聚糖酶的合成由纤维素、纤维二糖和水杨苷诱导。在纤维二糖上生长期间诱导的合成受到抑制，尽管葡萄糖并未完全阻遏。当细胞在不溶性或可溶性聚β-葡糖苷聚合物上生长时，外切葡聚糖酶活性增强。通过向微晶底物中添加越来越多的纤维二糖，活性逐渐受到抑制。
