秀丽隐杆线虫的弹道式转变
Ballistic transformation of Caenorhabditis elegans.
作者信息
Wilm T, Demel P, Koop H U, Schnabel H, Schnabel R
机构信息
Max Planck Institut für Biochemie, 82152, Martinsried, Germany.
出版信息
Gene. 1999 Mar 18;229(1-2):31-5. doi: 10.1016/s0378-1119(99)00043-8.
Abstract
A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system.
摘要
本文描述了一种转化线虫秀丽隐杆线虫的新方法。与金颗粒共沉淀的DNA通过氦束射向线虫。转化的线虫要么通过显性可见标记来鉴定，要么通过条件致死系统来筛选。
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