Bollmann R, Steinau H U, Bosse A
Institut für Pathologie, Bonn-Duisdorf.
Verh Dtsch Ges Pathol. 1998;82:351-7.
Malignant neoplasms are associated with chromosomal aberrations. This phenomenon called aneuploidy can be substantiated by interactive DNA cytometry and is accepted as a major prognostic parameter in tumor biology and oncology. In a retrospective study 114 non-infantile soft tissue tumors were analysed by DNA cytometry. The results were compared with classical morphological parameters. As a marker for malignancy in soft tissue lesions, DNA aneuploidy substantiated by cytometry has a sensitivity of 94% and a specifity of 82%: 50 of 53 malignant soft tissue tumors were DNA aneuploid; from 61 non-sarcomatous soft tissue tumors 11 were found to be aneuploid. These lesions were classified as intermediate dignity. No aneuploid tumor could be found among 30 histologically benign soft tissue tumors. The positive predictive value of DNA cytometry was 82%: of 61 aneuploid soft tissue tumors 11 were non-sarcomatous tumors with intermediate dignity. The negative predictive value of DNA cytometry was 97%: from 53 non-aneuploid soft tissue tumors only three were found to be sarcomas. Malignant soft tissue tumors demonstrate a significantly higher DNA-index (DI) and DNA grade of malignancy (DNA MG) than benign lesions. Our results show that DNA ploidy may serve as an additional parameter in problematic histological dignity assessments of soft tissue tumors. The histopathological grading levels of sarcomas show significantly different DI and DNA MG, so that DNA cytometry enables substantiated grading of soft tissue.
恶性肿瘤与染色体畸变有关。这种被称为非整倍体的现象可以通过交互式DNA细胞计数法得到证实，并且被公认为肿瘤生物学和肿瘤学中的一个主要预后参数。在一项回顾性研究中，对114例非婴儿软组织肿瘤进行了DNA细胞计数分析。将结果与经典形态学参数进行了比较。作为软组织病变恶性程度的标志物，通过细胞计数法证实的DNA非整倍体的敏感性为94%，特异性为82%：53例恶性软组织肿瘤中有50例为DNA非整倍体；61例非肉瘤性软组织肿瘤中有11例被发现为非整倍体。这些病变被归类为中等恶性程度。在30例组织学上良性的软组织肿瘤中未发现非整倍体肿瘤。DNA细胞计数法的阳性预测值为82%：在61例非整倍体软组织肿瘤中，11例为中等恶性程度的非肉瘤性肿瘤。DNA细胞计数法的阴性预测值为97%：在53例非非整倍体软组织肿瘤中，仅发现3例为肉瘤。恶性软组织肿瘤的DNA指数（DI）和DNA恶性度分级（DNA MG）显著高于良性病变。我们的结果表明，DNA倍性可作为软组织肿瘤组织学恶性程度评估存在问题时的一个附加参数。肉瘤的组织病理学分级水平显示出显著不同的DI和DNA MG，因此DNA细胞计数法能够对软组织进行可靠的分级。
