Gaspar'ian A V, Zborovskaia I B, Mukeriia A F, Tatosian A G, Zaridze D G
Institute of Carcinogenesis, Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
Genetika. 1998 Nov;34(11):1537-41.
Data on allelotyping of minisatellite sequence within the Hras1 protooncogene locus in 60 patients with lung adenocarcinoma (LAC) are presented. Allele distribution was analyzed with respect to the effect of tobacco smoke carcinogens (smoking factor). Results were compared with the analogous data obtained for patients with squamous-cell-carcinoma and for individuals without cancer. In contrast to the patients with squamous cell-carcinoma, the frequency of the Hras1 locus rare alleles in patients with lung-adenocarcinoma was higher than in individuals without cancer. More striking difference between the latter groups was demonstrated for nonsmoking patients (17.6% versus 2.7% of healthy individuals, P = 0.0005). In smoking LAC patients, higher frequencies of the common a4 allele (2.5 kb in size under the MspI/HpaII digestion) were found. Our findings point to the combined effect of endogenous and exogenous factors on the development of lung adenocarcinomas in humans. In this work, we discuss the possible mechanism of association between the rare minisatellite alleles and the predisposition to lung cancer.
本文呈现了60例肺腺癌（LAC）患者原癌基因Hras1位点内微卫星序列的等位基因分型数据。针对烟草烟雾致癌物（吸烟因素）的影响分析了等位基因分布。将结果与鳞状细胞癌患者及无癌个体的类似数据进行了比较。与鳞状细胞癌患者不同，肺腺癌患者中Hras1位点稀有等位基因的频率高于无癌个体。对于不吸烟患者，后两组之间的差异更为显著（分别为健康个体的17.6%和2.7%，P = 0.0005）。在吸烟的LAC患者中，发现常见的a4等位基因（经MspI/HpaII酶切后大小为2.5 kb）频率更高。我们的研究结果表明内源性和外源性因素对人类肺腺癌发生具有联合作用。在这项工作中，我们讨论了稀有微卫星等位基因与肺癌易感性之间关联的可能机制。
