Anderson S E, Bautista S C, Remington J S
Clin Exp Immunol. 1976 Dec;26(3):375-80.
The requirement for specificity of antibody-dependent inhibition or killing of intracellular Toxoplasma gondii trophozoites by normal mouse peritoneal macrophages was evaluated in vitro using light microscopy and autoradiography. Anti-toxoplasma antibody in the presence of 'accessory factor' rendered extracellular T. gondii trophozoites non-viable and non-infectious for cells, whereas exposure of extracellular trophozoites to heat-inactivated immune serum did not appear to damage the parasites. Although pretreatment of extracellular trophozoites with heat-inactivated immune serum neither diminished nor prevented infection of normal mouse peritoneal macrophages, it did confer upon macrophages the ability to inhibit or kill the organisms once they were intracellular. In contrast, pretreatment of trophozoites with either heat-inactivated normal or Besnoitia jellisoni immune serum did not enable normal macrophages to inhibit or kill T. gondii; rather, such organisms multiplied intracellularly in normal macrophages. Thus, pretreatment with specific antibody alone prepared T. gondii trophozoites for intracellular destruction by normal mouse peritoneal macrophages. These results suggest that spesific antibody acting in concert with normal macrophages may play a role in controlling infection with T. gondii.
利用光学显微镜和放射自显影技术在体外评估了正常小鼠腹腔巨噬细胞对细胞内刚地弓形虫滋养体进行抗体依赖性抑制或杀伤时对特异性的要求。在“辅助因子”存在的情况下，抗弓形虫抗体可使细胞外的刚地弓形虫滋养体失去活力且对细胞无感染性，而将细胞外滋养体暴露于热灭活的免疫血清中似乎不会损害寄生虫。虽然用热灭活的免疫血清对细胞外滋养体进行预处理既不会减少也不会阻止正常小鼠腹腔巨噬细胞的感染，但它确实赋予了巨噬细胞一旦滋养体进入细胞内就抑制或杀伤这些生物体的能力。相比之下，用热灭活的正常血清或耶氏贝诺孢子虫免疫血清对滋养体进行预处理并不能使正常巨噬细胞抑制或杀伤刚地弓形虫；相反，这些生物体在正常巨噬细胞内会增殖。因此，仅用特异性抗体进行预处理可使刚地弓形虫滋养体被正常小鼠腹腔巨噬细胞进行细胞内破坏。这些结果表明，特异性抗体与正常巨噬细胞协同作用可能在控制刚地弓形虫感染中发挥作用。
