Lin L C, Kuo Y C, Chou C J
National Research Institute of Chinese Medicine, Shih-Pai, Taipei 11221, Taiwan, Republic of China.
J Nat Prod. 1999 Mar;62(3):405-8. doi: 10.1021/np980153o.
From the ethanolic extract of Dichrocephala bicolor eight compounds-4,5-dicaffeoyl quinic acid (1); 3,4-dicaffeoyl quinic acid (2); 3,5-dicaffeoyl quinic acid (3); ethyl 4,5-dicaffeoyl quinate (4); methyl 3,5-dicaffeoyl quinate (5); 5-caffeoyl quinic acid (6); caffeic acid (7); and quercetin-3-O-rutinoside (8)-were isolated and identified. All of them were selected for immunopharmacological activity testing. Human mononuclear cells (HMNC) were used as target cells. Cell proliferation was determined by 3H-thymidine uptake. Compounds 2 and 6 potently enhanced HMNC proliferation and interferon-gamma production. Enhancement mechanisms may involve the increase of cytokines production.
从二色金盏菊的乙醇提取物中分离并鉴定出了8种化合物，分别是4,5-二咖啡酰奎宁酸（1）；3,4-二咖啡酰奎宁酸（2）；3,5-二咖啡酰奎宁酸（3）；4,5-二咖啡酰奎宁酸乙酯（4）；3,5-二咖啡酰奎宁酸甲酯（5）；5-咖啡酰奎宁酸（6）；咖啡酸（7）；以及槲皮素-3-O-芸香糖苷（8）。所有这些化合物都被选来进行免疫药理学活性测试。人单核细胞（HMNC）用作靶细胞。通过3H-胸腺嘧啶核苷摄取来测定细胞增殖。化合物2和6显著增强了HMNC的增殖以及γ-干扰素的产生。增强机制可能涉及细胞因子产生的增加。
