Università di Bologna, Dipartimento di Biologia Evoluzionistica Sperimentale, Bologna, Italy.
Fitoterapia. 2011 Oct;82(7):950-4. doi: 10.1016/j.fitote.2011.05.008. Epub 2011 May 23.
3,5-O-dicaffeoyl-4-O-malonilquinic acid (1) (irbic acid) has been isolated for the first time from cell cultures of Centella asiatica and till now it has never been reported to be present in the intact plant. Evidence of its structure was obtained by spectroscopic analyses (MS/NMR). Besides 1, cell cultures produce also the known 3,5-O-dicaffeoylquinic acid, chlorogenic acid, and the triferulic acid 2 (4-O-8'/4'-O-8″-didehydrotriferulic acid). Biological activities were evaluated for compound 1, which showed to have a strong radical scavenging capacity, together with a high inhibitory activity on collagenase. This suggests a possible utilization of this substance as a topical agent to reduce the skin ageing process.
3,5-O-二咖啡酰基-4-O-丙二酰奎宁酸（1）（irbic 酸）首次从积雪草细胞培养物中分离出来，到目前为止，它从未在完整植物中被报道存在。通过光谱分析（MS/NMR）获得了其结构的证据。除了 1，细胞培养物还产生已知的 3,5-O-二咖啡酰奎宁酸、绿原酸和三咖啡酸 2（4-O-8'/4'-O-8″-去氢三咖啡酸）。对化合物 1 进行了生物活性评价，结果表明其具有很强的自由基清除能力，同时对胶原酶有很高的抑制活性。这表明该物质可作为一种局部制剂，用于减少皮肤老化过程。
