通过大肠杆菌乳糖通透酶的半胱氨酸扫描诱变推导磷酸烯醇丙酮酸：糖磷酸转移酶系统中与IIAGlc结合的蛋白质上的共有结合序列。
Deduction of consensus binding sequences on proteins that bind IIAGlc of the phosphoenolpyruvate:sugar phosphotransferase system by cysteine scanning mutagenesis of Escherichia coli lactose permease.
作者信息
Sondej M, Sun J, Seok Y J, Kaback H R, Peterkofsky A
机构信息
Laboratory of Biochemical Genetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3525-30. doi: 10.1073/pnas.96.7.3525.
Abstract
Mediated by the protein IIAGlc, the phosphoenolpyruvate:sugar phosphotransferase system plays a role in the regulation of activity of other sugar transport systems in Escherichia coli. By using a direct binding assay, a collection of single-Cys replacement mutants in cytoplasmic loops of lactose permease were evaluated for their capacity to bind IIAGlc. Selected Cys replacements in loops IV/V or VI/VII result in loss of binding activity. Analysis of the mutagenesis results together with multiple sequence alignments of a family of proteins that interacts with IIAGlc provides the basis for developing two regions of consensus sequence in those partner proteins necessary for binding to IIAGlc. The requirement for two interaction regions is interpreted in the regulatory framework of a substrate-dependent conformational change that brings those two regions into an orientation optimal for binding IIAGlc.
摘要
磷酸烯醇丙酮酸
糖磷酸转移酶系统由蛋白IIAGlc介导，在大肠杆菌中其他糖转运系统活性的调节中发挥作用。通过直接结合试验，对乳糖通透酶细胞质环中一系列单半胱氨酸替代突变体结合IIAGlc的能力进行了评估。在环IV/V或VI/VII中选择的半胱氨酸替代导致结合活性丧失。对诱变结果的分析以及与IIAGlc相互作用的一类蛋白质的多序列比对，为在那些与IIAGlc结合所必需的伴侣蛋白中开发两个共有序列区域提供了基础。对两个相互作用区域的需求在底物依赖性构象变化的调节框架中得到解释，这种变化使这两个区域处于最适合结合IIAGlc的方向。
相似文献
1
Deduction of consensus binding sequences on proteins that bind IIAGlc of the phosphoenolpyruvate:sugar phosphotransferase system by cysteine scanning mutagenesis of Escherichia coli lactose permease.通过大肠杆菌乳糖通透酶的半胱氨酸扫描诱变推导磷酸烯醇丙酮酸：糖磷酸转移酶系统中与IIAGlc结合的蛋白质上的共有结合序列。
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3525-30. doi: 10.1073/pnas.96.7.3525.
2
Topology of allosteric regulation of lactose permease.乳糖通透酶的变构调节拓扑结构
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13515-9. doi: 10.1073/pnas.94.25.13515.
3
Topography of the surface of the Escherichia coli phosphotransferase system protein enzyme IIAglc that interacts with lactose permease.与乳糖通透酶相互作用的大肠杆菌磷酸转移酶系统蛋白IIAglc的表面拓扑结构。
Biochemistry. 2000 Mar 21;39(11):2931-9. doi: 10.1021/bi9919596.
4
Binding of enzyme IIAGlc, a component of the phosphoenolpyruvate:sugar phosphotransferase system, to the Escherichia coli lactose permease.磷酸烯醇丙酮酸：糖磷酸转移酶系统的一个组分——酶IIAGlc与大肠杆菌乳糖通透酶的结合。
Biochemistry. 2002 Apr 30;41(17):5556-65. doi: 10.1021/bi011990j.
5
Involvement of the central loop of the lactose permease of Escherichia coli in its allosteric regulation by the glucose-specific enzyme IIA of the phosphoenolpyruvate-dependent phosphotransferase system.大肠杆菌乳糖通透酶的中央环在磷酸烯醇式丙酮酸依赖性磷酸转移酶系统的葡萄糖特异性酶IIA对其变构调节中的作用。
J Bacteriol. 1996 Oct;178(20):6082-6. doi: 10.1128/jb.178.20.6082-6086.1996.
6
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off.大肠杆菌乳糖通透酶中螺旋IV及相邻环的半胱氨酸扫描诱变：Glu126和Arg144至关重要。关闭。
Biochemistry. 1997 Nov 25;36(47):14284-90. doi: 10.1021/bi972314d.
7
Characterization of a lactose permease mutant that binds IIAGlc in the absence of ligand.一种在无配体情况下结合IIAGlc的乳糖通透酶突变体的特性分析。
Biochemistry. 2003 Aug 5;42(30):9153-9. doi: 10.1021/bi034406a.
8
Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus.嗜热链球菌乳糖转运蛋白IIA结构域的磷酸化及功能特性
J Bacteriol. 1999 Jan;181(2):632-41. doi: 10.1128/JB.181.2.632-641.1999.
9
Regulation of glycerol and maltose uptake by the IIAGlc-like domain of IINag of the phosphotransferase system in Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2中磷酸转移酶系统的IINag的IIAGlc样结构域对甘油和麦芽糖摄取的调控
Mol Gen Genet. 1995 Jul 28;248(2):236-41. doi: 10.1007/BF02190806.
10
Cysteine-scanning mutagenesis of putative helix VII in the lactose permease of Escherichia coli.大肠杆菌乳糖通透酶中假定的螺旋VII的半胱氨酸扫描诱变
Biochemistry. 1994 Jul 5;33(26):8074-81. doi: 10.1021/bi00192a012.
引用本文的文献
1
Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.翻转开关：细菌中膜转运蛋白活性的动态调节。
Microbiology (Reading). 2023 Nov;169(11). doi: 10.1099/mic.0.001412.
2
The molecular mechanism for carbon catabolite repression of the chitin response in Vibrio cholerae.霍乱弧菌中几丁质反应的碳分解代谢物阻遏的分子机制。
PLoS Genet. 2023 May 12;19(5):e1010767. doi: 10.1371/journal.pgen.1010767. eCollection 2023 May.
3
Control of Transposon-Mediated Directed Mutation by the Escherichia coli Phosphoenolpyruvate:Sugar Phosphotransferase System.大肠杆菌磷酸烯醇式丙酮酸：糖磷酸转移酶系统对转座子介导的定向突变的控制
J Mol Microbiol Biotechnol. 2015;25(2-3):226-33. doi: 10.1159/000375375. Epub 2015 Jul 9.
4
Thermodynamic mechanism for inhibition of lactose permease by the phosphotransferase protein IIAGlc.磷酸转移酶蛋白IIAGlc抑制乳糖通透酶的热力学机制。
Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2407-12. doi: 10.1073/pnas.1500891112. Epub 2015 Feb 9.
5
Multiple sugar: phosphotransferase system permeases participate in catabolite modification of gene expression in Streptococcus mutans.多种糖：磷酸转移酶系统通透酶参与变形链球菌中基因表达的分解代谢修饰。
Mol Microbiol. 2008 Oct;70(1):197-208. doi: 10.1111/j.1365-2958.2008.06403.x. Epub 2008 Aug 11.
6
How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.磷酸转移酶系统相关蛋白磷酸化如何调节细菌中的碳水化合物代谢。
Microbiol Mol Biol Rev. 2006 Dec;70(4):939-1031. doi: 10.1128/MMBR.00024-06.
7
Negative control of rpoS expression by phosphoenolpyruvate: carbohydrate phosphotransferase system in Escherichia coli.磷酸烯醇式丙酮酸：碳水化合物磷酸转移酶系统对大肠杆菌中rpoS表达的负调控
J Bacteriol. 2001 Jan;183(2):520-7. doi: 10.1128/JB.183.2.520-527.2001.
8
The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion.转运蛋白主要易化子超家族的中央细胞质环决定了有效的膜插入。
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8938-43. doi: 10.1073/pnas.140224497.
本文引用的文献
1
Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins.半胱氨酸扫描诱变：一种研究多跨膜蛋白结构与功能关系的新方法。
FASEB J. 1998 Oct;12(13):1281-99. doi: 10.1096/fasebj.12.13.1281.
2
The substrate-binding site in the lactose permease of Escherichia coli.大肠杆菌乳糖通透酶中的底物结合位点。
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9802-7. doi: 10.1073/pnas.95.17.9802.
3
Structural studies of the Escherichia coli signal transducing protein IIAGlc: implications for target recognition.大肠杆菌信号转导蛋白IIAGlc的结构研究：对靶点识别的启示
Biochemistry. 1997 Dec 23;36(51):16087-96. doi: 10.1021/bi971999e.
4
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off.大肠杆菌乳糖通透酶中螺旋IV及相邻环的半胱氨酸扫描诱变：Glu126和Arg144至关重要。关闭。
Biochemistry. 1997 Nov 25;36(47):14284-90. doi: 10.1021/bi972314d.
5
Topology of allosteric regulation of lactose permease.乳糖通透酶的变构调节拓扑结构
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13515-9. doi: 10.1073/pnas.94.25.13515.
6
Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.多跨膜蛋白中的螺旋堆积：大肠杆菌乳糖通透酶
Curr Opin Struct Biol. 1997 Aug;7(4):537-42. doi: 10.1016/s0959-440x(97)80119-4.
7
Involvement of the central loop of the lactose permease of Escherichia coli in its allosteric regulation by the glucose-specific enzyme IIA of the phosphoenolpyruvate-dependent phosphotransferase system.大肠杆菌乳糖通透酶的中央环在磷酸烯醇式丙酮酸依赖性磷酸转移酶系统的葡萄糖特异性酶IIA对其变构调节中的作用。
J Bacteriol. 1996 Oct;178(20):6082-6. doi: 10.1128/jb.178.20.6082-6086.1996.
8
Cysteine-scanning mutagenesis of helix VI and the flanking hydrophilic domains on the lactose permease of Escherichia coli.大肠杆菌乳糖通透酶螺旋VI及侧翼亲水区的半胱氨酸扫描诱变
Biochemistry. 1996 Apr 23;35(16):5333-8. doi: 10.1021/bi953068d.
9
Bacterial adenylyl cyclases.细菌腺苷酸环化酶
Prog Nucleic Acid Res Mol Biol. 1993;44:31-65. doi: 10.1016/s0079-6603(08)60216-0.
10
Structure of the regulatory complex of Escherichia coli IIIGlc with glycerol kinase.大肠杆菌IIIGlc与甘油激酶的调节复合物结构
Science. 1993 Jan 29;259(5095):673-7.
Zotero 插件