采用高效液相色谱-同位素稀释质谱法（IDMS）测定尿液中的麦角酰二乙胺（LSD）。
The determination of lysergide (LSD) in urine by high-performance liquid chromatography-isotope dilution mass spectrometry (IDMS).
作者信息
White S A, Kidd A S, Webb K S
机构信息
Laboratory of the Government Chemist, Teddington, Middlesex, England.
出版信息
J Forensic Sci. 1999 Mar;44(2):375-9.
The use of isotope dilution mass spectrometry (IDMS) has been investigated for the forensic confirmation of lysergic acid diethylamide (LSD) in urine by LC-MS. The advantages of using a deuterated analog of LSD as an internal standard over methysergide are discussed. This study includes a comparison of the electrospray mass spectra of LSD, LSD-d3 and methysergide, and discusses the choice of suitable ions for use in selected ion monitoring (SIM) mode. An IDMS method is presented for the LC-MS confirmation of LSD in urine, with a limit of quantification (LOQ) of 0.5 ng/mL, reflecting the forensic requirement at this laboratory. Under some circumstances the LOQ can be improved to 0.1 ng/mL. This method is linear in the range tested (up to 10 ng/mL LSD in urine) and has been validated in terms of accuracy and precision.
已对采用同位素稀释质谱法（IDMS）通过液相色谱-质谱联用（LC-MS）对尿液中麦角酸二乙酰胺（LSD）进行法医鉴定展开了研究。讨论了使用LSD的氘代类似物作为内标相较于甲基麦角新碱的优势。本研究包括对LSD、LSD-d3和甲基麦角新碱的电喷雾质谱进行比较，并讨论了在选择离子监测（SIM）模式下使用的合适离子的选择。提出了一种用于LC-MS确认尿液中LSD的IDMS方法，定量限（LOQ）为0.5 ng/mL，反映了该实验室的法医要求。在某些情况下，定量限可提高至0.1 ng/mL。该方法在所测试的范围内（尿液中LSD高达10 ng/mL）呈线性，并且已在准确度和精密度方面得到验证。
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