White S A, Catterick T, Harrison M E, Johnston D E, Reed G D, Webb K S
Laboratory of the Government Chemist, Teddington, Middlesex, UK.
J Chromatogr B Biomed Sci Appl. 1997 Feb 21;689(2):335-40. doi: 10.1016/s0378-4347(96)00344-1.
A quantitative method which avoids derivatisation is described for the determination of lysergide (LSD) levels in urine. Sample preparation included addition of methysergide as an internal standard followed by solid-phase extraction. LSD was analysed on a system consisting of a C18 stationary phase and a mobile phase of 0.1 M acetate buffer pH 8.0-acetonitrile-triethylamine (75:25:0.25, v/v). LSD was detected by electrospray ionisation mass spectrometry with selected ion monitoring. The quantification limit was 0.5 ng/ml and the method was linear up to 10 ng/ml of LSD in urine.
