Iwaki H, Sakamoto A, Tanaka S
Second Department of Surgery, Nippon Medical School, Japan.
Nihon Ika Daigaku Zasshi. 1999 Feb;66(1):15-20. doi: 10.1272/jnms.66.15.
In clinical transplantation and cardiovascular surgery, cold preservation is usually used because it is a simple method. However, the established temperature is by no means exact. The aim of this study was to find the optimum storage temperature for preservation of the vasculature by observing the pharmacological endothelium and smooth muscle response. The thoracic aorta of 36 male Wister rats were studied in organ baths: as fresh control after 24 hours, 48 hours and 72 hours of storage at 0.5 degree C, 4 degrees C and 8 degrees C in Krebs-Henseleit bicarbonate (KHB) solution. Acetylcholine (Ach) was used to elicit endothelium-dependent relaxation, and sodium nitroprusside (SNP) to elicit smooth muscle-dependent relaxation. The contractility caused by Phenylephrine (Ph) was influenced by time but before 48 hours it was not influenced by preservation temperature. Significant responsive deterioration by Ach and SNP was seen after 24 hours of storage at 0.5 degree C as compared with storage at 4 degrees C. The endothelium-dependent relaxing function and smooth muscle-dependent relaxing function were best preserved at 4 degrees C and 8 degrees C. These results indicate that precise temperature control is necessary for vessel preservation in clinical situations.
在临床移植和心血管外科手术中，通常采用冷保存法，因为它是一种简单的方法。然而，设定的温度绝非精确。本研究的目的是通过观察药理学上的内皮和平滑肌反应，找到保存脉管系统的最佳储存温度。在器官浴中对36只雄性Wistar大鼠的胸主动脉进行了研究：在0.5摄氏度、4摄氏度和8摄氏度的Krebs-Henseleit碳酸氢盐（KHB）溶液中储存24小时、48小时和72小时后，作为新鲜对照。使用乙酰胆碱（Ach）引发内皮依赖性舒张，使用硝普钠（SNP）引发平滑肌依赖性舒张。去氧肾上腺素（Ph）引起的收缩性受时间影响，但在48小时之前不受保存温度的影响。与在4摄氏度储存相比，在0.5摄氏度储存24小时后，Ach和SNP出现明显的反应性恶化。内皮依赖性舒张功能和平滑肌依赖性舒张功能在4摄氏度和8摄氏度时保存得最好。这些结果表明，在临床情况下，精确的温度控制对于血管保存是必要的。
