通过小血管肌动描记法研究大鼠肠系膜阻力动脉冷藏后的血管功能保存情况。

Preservation of vascular function in rat mesenteric resistance arteries following cold storage, studied by small vessel myography.

作者信息

McIntyre C A, Williams B C, Lindsay R M, McKnight J A, Hadoke P W

机构信息

Department of Medicine, Western General Hospital, Edinburgh, Scotland.

出版信息

Br J Pharmacol. 1998 Apr;123(8):1555-60. doi: 10.1038/sj.bjp.0701768.

DOI:10.1038/sj.bjp.0701768

PMID:9605561

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1565325/

Abstract

The use of isolated blood vessels to investigate the physiological and pharmacological control of the vasculature is limited by the requirement to use freshly isolated vessels. Hence, the aim of this study was to determine whether vascular smooth muscle and endothelial cell function could be preserved in resistance arteries by storing them in physiological salt solution (PSS) at 4 degrees C. 2. Third order mesenteric resistance arteries (mean internal diameter 237+/-6 microm) were dissected from the mesenteric bed of male Cob-Wistar rats. The vessel segments were mounted in a small vessel myograph for measurement of isometric tension, and equilibrated at their optimum resting force. Contractile responses to noradrenaline (NA; 1 x 10(-9) - 3 x 10(-5) M), phenylephrine (PE, 1 x 10(-9)-3 x 10(-5) M), potassium chloride (KCI; 2.5-140 mM) and endothelin (ET-1, 1 x 10(-11)-3 x 10(-7) M) and relaxant responses to acetylcholine (ACh; 1 x 10(-9) - 3 x 10(-5) M) and 3-morpholinosydnonimine (SIN-1; 1 x 10(-9) - 1 x 10(-4) M) were obtained in arteries, immediately after dissection (day 0) and following one to four days storage (day 1-day 4). 3. All arteries produced concentration-dependent contractions in response to each of the vasoconstrictors. There were no significant differences in the magnitude or sensitivity (pD2) of the vasoconstrictor responses between fresh and stored vessels. 4. Arteries precontracted with NA to approximately 80% of the maximum response, relaxed in a concentration-dependent manner in response to ACh and SIN-1. Vessel storage for up to three days resulted in no change in response to ACh or SIN-1. 5. Vessels analysed after four days of storage demonstrated a significant increase in sensitivity to ACh and SIN-1 (-logIC50 (M) values; ACh; day 0, 7.46+/-0.13 vs day 4, 7.97+/-0.11, P<0.01 and SIN-1; day 0, 4.87+/-0.10 vs day 4, 5.52+/-0.08, P<0.01). There was also a significant increase in the maximum relaxant response to ACh after four days of storage (% relaxation; day 0, 92.65+/-2.84 vs day 4, 100.36+/-0.36, P<0.05). 6. These results demonstrate that small resistance arteries remain viable if stored in PSS at 4 degrees C for up to four days, with no loss in endothelial cell function. The altered sensitivity to the vasodilators on day 4 suggests that vessels should only be stored for up to three days following dissection for analysis of functional responses.

摘要

使用分离的血管来研究血管系统的生理和药理控制受到需要使用新鲜分离血管的限制。因此，本研究的目的是确定通过将阻力动脉储存在4℃的生理盐溶液（PSS）中，血管平滑肌和内皮细胞功能是否能够得以保留。2. 从雄性Cob-Wistar大鼠的肠系膜床中分离出三级肠系膜阻力动脉（平均内径237±6微米）。将血管段安装在小型血管肌张力描记仪中以测量等长张力，并在其最佳静息力下平衡。在新鲜分离（第0天）以及储存一至四天（第1天至第4天）后，测定动脉对去甲肾上腺素（NA；1×10⁻⁹ - 3×10⁻⁵M）、去氧肾上腺素（PE，1×10⁻⁹ - 3×10⁻⁵M）、氯化钾（KCl；2.5 - 140mM）和内皮素（ET-1，1×10⁻¹¹ - 3×10⁻⁷M）的收缩反应，以及对乙酰胆碱（ACh；1×10⁻⁹ - 3×10⁻⁵M）和3-吗啉代辛二亚胺（SIN-1；1×10⁻⁹ - 1×10⁻⁴M）的舒张反应。3. 所有动脉对每种血管收缩剂均产生浓度依赖性收缩。新鲜血管和储存血管之间血管收缩剂反应的幅度或敏感性（pD2）无显著差异。4. 用NA预收缩至最大反应约80%的动脉，对ACh和SIN-1呈浓度依赖性舒张。储存长达三天的血管对ACh或SIN-1的反应无变化。5. 储存四天后分析的血管对ACh和SIN-1的敏感性显著增加（-logIC50（M）值；ACh：第0天，7.46±0.13对第4天，7.97±0.11，P<0.01；SIN-1：第0天，4.87±0.10对第4天，5.52±0.08，P<0.01）。储存四天后对ACh的最大舒张反应也显著增加（舒张百分比；第0天，92.65±2.84对第4天，100.36±0.36，P<0.05）。6. 这些结果表明，小阻力动脉如果在4℃的PSS中储存长达四天仍保持活力，内皮细胞功能无损失。第4天对血管舒张剂敏感性的改变表明，为分析功能反应，血管在分离后仅应储存长达三天。