Joste N E, Sax P E, Pieciak W S
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
Acta Cytol. 1999 Mar-Apr;43(2):98-103. doi: 10.1159/000330959.
To compare stains in preparations of bile in a patient with AIDS and microsporidial cholangitis.
Bile was obtained from a 30-year-old male with AIDS and symptoms of cholangitis. Comparative staining of the specimen was performed using a formalin-fixed preparation stained with Chromotrope 2R stain and with alcohol-fixed preparations stained with Gram and Giemsa stain and Diff-Quik. An alcohol-fixed ThinPrep slide was stained with Papanicolaou stain.
Diagnostic microsporidia spores were detected under oil immersion using Papanicolaou, Chromotrope 2R, Giemsa and Gram stain. The Diff-Quik-stained preparation also revealed microsporidia but with suboptimal morphology.
Detection of microsporidia in bile can be achieved using several different stains routinely available to cytologists, most optimally with alcohol-fixed Papanicolaou- or Giemsa-stained preparations or with Chromotrope 2R stain, which is available in parasitology laboratories. These findings should be applicable to fluids from other body sites with this emerging pathogen in AIDS.
比较艾滋病合并微孢子虫胆管炎患者胆汁制剂中的染色情况。
胆汁取自一名30岁患有艾滋病且有胆管炎症状的男性。对标本进行对比染色，使用经铬变素2R染色的福尔马林固定制剂，以及经革兰氏染色、吉姆萨染色和Diff-Quik染色的酒精固定制剂。一张酒精固定的ThinPrep玻片用巴氏染色法染色。
在油镜下，使用巴氏染色法、铬变素2R染色法、吉姆萨染色法和革兰氏染色法均检测到诊断性微孢子虫孢子。Diff-Quik染色制剂也显示出微孢子虫，但形态欠佳。
使用细胞学家常规可用几种不同的染色方法可以在胆汁中检测到微孢子虫，最理想的是使用酒精固定的巴氏染色或吉姆萨染色制剂，或使用寄生虫学实验室可用的铬变素2R染色法。这些发现应适用于艾滋病患者体内其他部位含有这种新出现病原体的体液。
