Belloni A S, Andreis P G, Meneghelli V, Champion H C, Kadowitz P J, Coy D H, Murphy W A, Nussdorfer G G
Department of Human Anatomy and Physiology, Padua University, Italy.
Endocr Res. 1999 Feb;25(1):29-34. doi: 10.1080/07435809909066127.
Frozen sections of normal adrenal glands, obtained from patients undergoing unilateral nephrectomy for kidney cancer, were labeled in vitro with human [125I]ADM(1-52). Autoradiography showed the presence of abundant ADM binding sites in the zona glomerulosa (ZG) and the outermost portion of the zona fasciculata, which were completely displaced by the addition of an excess of cold ADM(1-52). Calcitonin gene-related peptide (CGRP) and the non-selective ligand of the CGRP-receptor subtypes 1 and 2 CGRP(8-37) eliminated [125I]ADM(1-52) binding in the ZG, while the selective ligand of CGRP receptor subtype 2 [Cys(acm)2,7]-CGRP and CGRP(1-8) were ineffective. These findings confirm the presence of ADM binding sites in the human ZG, and provide the first morphological evidence that ADM and CGRP interact with a common receptor of the CGRP1 subtype.
取自因肾癌接受单侧肾切除术患者的正常肾上腺冰冻切片，在体外用人[125I]ADM(1 - 52)进行标记。放射自显影显示，在肾上腺皮质球状带（ZG）和束状带最外层存在丰富的ADM结合位点，加入过量的冷ADM(1 - 52)可使其完全被取代。降钙素基因相关肽（CGRP）以及CGRP受体亚型1和2的非选择性配体CGRP(8 - 37)可消除ZG中[125I]ADM(1 - 52)的结合，而CGRP受体亚型2的选择性配体[Cys(acm)2,7]-CGRP和CGRP(1 - 8)则无效。这些发现证实了人ZG中存在ADM结合位点，并首次提供了形态学证据，表明ADM和CGRP与CGRP1亚型的共同受体相互作用。
