Belloni A S, Meneghelli V, Champion H C, Murray W A, Coy D H, Kadowitz P J, Nussdorfer G G
Department of Anatomy, University of Padua, Italy.
Peptides. 1998;19(9):1581-4. doi: 10.1016/s0196-9781(98)00111-9.
Frozen sections of normal adrenal glands, obtained from patients undergoing unilateral nephrectomy for kidney cancer, were labeled in vitro with human [125I]ADM(1-52). Autoradiography and quantitative densitometry showed the presence of abundant ADM(1-52) binding sites in both zona glomerulosa (ZG) and capsular vessels, which were displaced with about the same efficiency by cold ADM(1-52) and rat ADM(1-50). The selective calcitonin gene-related peptide type 1 (CGRPI) ligand CGRP(8-37) eliminated, although less efficiently than ADMs, [125I]ADM(1-52) binding in the ZG, but not in the capsular vessels. These findings suggest the existence of different receptor subtypes for ADM in the human adrenal cortex. The CGRP(8-37)-sensitive receptors located in the ZG may mediate the well-known inhibitory effect of ADM on aldosterone secretion, while the CGRP(8-37)-insensitive receptors present in the capsular vessel may be involved in the ADM-induced rise in adrenal blood flow.
从因肾癌接受单侧肾切除术的患者获取的正常肾上腺冰冻切片，在体外用人[125I]ADM(1 - 52)进行标记。放射自显影和定量密度测定显示，在球状带（ZG）和包膜血管中均存在丰富的ADM(1 - 52)结合位点，冷ADM(1 - 52)和大鼠ADM(1 - 50)以大致相同的效率使其发生位移。选择性降钙素基因相关肽1型（CGRPI）配体CGRP(8 - 37)可消除ZG中[125I]ADM(1 - 52)的结合，但其效率低于ADM，而在包膜血管中则无此作用。这些发现提示人肾上腺皮质中存在不同的ADM受体亚型。位于ZG中的CGRP(8 - 37)敏感受体可能介导了ADM对醛固酮分泌的众所周知的抑制作用，而包膜血管中存在的CGRP(8 - 37)不敏感受体可能参与了ADM诱导的肾上腺血流增加。
