McDowell A E, Harner R S, Pardue H L
Clin Chem. 1976 Nov;22(11):1862-7.
A rapid scanning vidicon spectrometer has been evaluated for the simultaneous determination of drugs in mixtures, without a separation step. Spectral data in the ultraviolet region are collected on-line with a small computer at repetition rates of 100 scans per second. Absorbance data at several wavelengths are processed by matrix equations to resolve them into the concentration of each component in each mixture. Results are reported for two-component mixtures of procainamide and N-acetylprocainamide in serum, for two-component aqueous mixtures of butabarbital and seconbarbital, and for seven-component aqueous mixtures of phenobarbital, diphenylhydantoin, aminophylline, acetaminophen, salicylamide, phenylbutazone, and secobarbital. We conclude that the computer-inter-faced vidicon spectrometer is a viable tool for simultaneous multicomponent determinations.
已对一种快速扫描光导摄像管光谱仪进行了评估，用于在无需分离步骤的情况下同时测定混合物中的药物。紫外区域的光谱数据通过小型计算机以每秒100次扫描的重复速率在线收集。通过矩阵方程处理几个波长处的吸光度数据，以将它们解析为每种混合物中各组分的浓度。报告了血清中普鲁卡因酰胺和N-乙酰普鲁卡因酰胺两组分混合物、布他比妥和司可巴比妥两组分水溶液混合物以及苯巴比妥、苯妥英、氨茶碱、对乙酰氨基酚、水杨酰胺、保泰松和司可巴比妥七组分水溶液混合物的结果。我们得出结论，计算机接口光导摄像管光谱仪是同时进行多组分测定的可行工具。
