Coutinho C M, van Leuven F, Araújo-Jorge T C
Departmento Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Parasitol Res. 1999 Apr;85(4):249-55. doi: 10.1007/s004360050543.
The aim of this work was to investigate the presence of alpha-macroglobulin (AM) in the heart of mice during acute experimental Chagas' disease and to study its localization as related to the presence of the parasite and/or to their antigens. Frozen heart tissue sections obtained from Swiss albino male mice at different days postinfection with Trypanosoma cruzi were examined for triple immunofluorescence in response to parasite antigen (green), AM (red), and nuclei (blue) from both cells. AM was found in the heart of all the infected animals studied. Parasites were seen arranged in nests inside heart muscle cells. Usually, AM staining corresponded in position to parasite nests and to their antigens spread in large areas of the myocardium. The most intense staining of AM was observed at days 9 and 11 postinfection, when the highest tissue-infection level occurs. These observations relate the presence of AM to that of T. cruzi antigen in the same areas of the inflamed myocardium of the chagasic animals.
这项工作的目的是研究急性实验性恰加斯病期间小鼠心脏中α-巨球蛋白（AM）的存在情况，并研究其定位与寄生虫及其抗原存在的关系。从感染克氏锥虫不同天数的瑞士白化雄性小鼠获取冷冻心脏组织切片，针对寄生虫抗原（绿色）、AM（红色）以及两种细胞的细胞核（蓝色）进行三重免疫荧光检查。在所研究的所有受感染动物的心脏中均发现了AM。可见寄生虫聚集在心肌细胞内形成巢状。通常，AM染色在位置上与寄生虫巢及其在心肌大面积区域扩散的抗原相对应。在感染后第9天和第11天观察到AM染色最为强烈，此时组织感染水平最高。这些观察结果表明，在恰加斯病动物炎症心肌的相同区域中，AM的存在与克氏锥虫抗原的存在相关。
