Frazer B A, Fried B, Fujino T, Sleckman B P
Department of Biology, Lafayette College, Easton, PA 18042, USA.
Parasitol Res. 1999 Apr;85(4):337-42. doi: 10.1007/s004360050558.
The RAG-2-deficient mouse, a strain of genetically altered mice lacking B- and T-lymphocytes, was used as a host for Echinostoma caproni. In all, 12 male RAG mice were exposed to 25 cysts each, and 12 served as uninfected controls. Mice were necropsied at 2 and 3 weeks postinfection (p.i.). The mean number+/-SE (9.7+/-2.4) of worms recovered from infected mice at 2 weeks p.i. was not significantly different from that recovered at 3 weeks p.i. (6.5+/-2.2). The intestinal circumference of infected RAG mice was significantly greater than that of the controls at 2 and 3 weeks p.i. A significant goblet cell hyperplasia occurred at 2 weeks p.i., but the response was not effective in eliminating worms from the RAG mice. The effect of a high cyst burden was examined by exposure of 8 RAG and 8 ICR mice to 100 cysts each. The body length and area and the oral sucker area of worms grown in RAG mice were significantly greater than those of worms grown in ICR mice. Worm recovery at up to 3 months p.i. was examined in RAG mice exposed to 25 cysts and necropsied every 2 weeks p.i. The mean worm recovery recorded at 2 weeks p.i. was significantly greater than that noted at 12 weeks p.i., at which time worm rejection from the RAG mouse host first occurred. The RAG mouse is a useful host for studies on E. caproni in a murine host that lacks B- and T-lymphocytes.
RAG-2基因缺陷小鼠是一种缺乏B淋巴细胞和T淋巴细胞的基因改造小鼠品系，被用作卡氏棘口吸虫的宿主。总共12只雄性RAG小鼠每只暴露于25个囊蚴，另有12只作为未感染对照。在感染后（p.i.）2周和3周对小鼠进行尸检。感染小鼠在感染后2周回收的蠕虫平均数量±标准误（9.7±2.4）与感染后3周回收的蠕虫数量（6.5±2.2）无显著差异。感染的RAG小鼠在感染后2周和3周时的肠周长显著大于对照组。感染后2周出现显著的杯状细胞增生，但这种反应未能有效清除RAG小鼠体内的蠕虫。通过让8只RAG小鼠和8只ICR小鼠每只暴露于100个囊蚴来研究高囊蚴负荷的影响。在RAG小鼠体内生长的蠕虫的体长、面积和口吸盘面积显著大于在ICR小鼠体内生长的蠕虫。对暴露于25个囊蚴且每2周进行一次尸检的RAG小鼠在感染后长达3个月的蠕虫回收率进行了检查。感染后2周记录的平均蠕虫回收率显著高于12周时的回收率，在12周时首次出现RAG小鼠宿主对蠕虫的排斥。RAG小鼠是在缺乏B淋巴细胞和T淋巴细胞的鼠宿主中研究卡氏棘口吸虫的有用宿主。
