McGhee J R, Kiyono H, Kubota M, Kawabata S, Miller C J, Lehner T, Imaoka K, Fujihashi K
Immunobiology Vaccine Center and Departments of Oral Biology and Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294-2170, USA.
J Infect Dis. 1999 May;179 Suppl 3:S480-4. doi: 10.1086/314807.
Simian immunodeficiency virus (SIV)-specific B cell responses and the Th1- or Th2-type profiles of cytokine expression were determined for rhesus macaques immunized with SIV antigens via the iliac lymph nodes (by use of a targeted lymph node [TLN] procedure) or orally with SIV p55gag plus cholera toxin (CT) as a mucosal adjuvant. Analysis of CD4+ T cells purified from SIV-stimulated peripheral blood mononuclear cells of immunized macaques revealed that Th2 cytokine production gradually increased after the second and third TLN immunization. Analysis of SIV-specific B cell responses revealed that peak SIV-specific IgA B cell responses followed the third TLN immunization and occurred during peak Th2-type T cell responses. Oral immunization of macaques with p55gag plus CT induced interferon-gamma-secreting Th1-type and select Th2-type cytokine-producing CD4+ T helper cells, which most likely accounted for the induction of p55-specific systemic IgG and mucosal IgA responses.
通过髂淋巴结（采用靶向淋巴结[TLN]程序）用SIV抗原免疫恒河猴，或口服SIV p55gag加霍乱毒素（CT）作为黏膜佐剂，测定了恒河猴针对猿猴免疫缺陷病毒（SIV）的B细胞反应以及细胞因子表达的Th1或Th2型特征。对从免疫猕猴的SIV刺激外周血单核细胞中纯化的CD4 + T细胞进行分析发现，在第二次和第三次TLN免疫后，Th2细胞因子的产生逐渐增加。对SIV特异性B细胞反应的分析表明，SIV特异性IgA B细胞反应的峰值出现在第三次TLN免疫之后，并发生在Th2型T细胞反应的高峰期。用p55gag加CT对猕猴进行口服免疫诱导了分泌干扰素-γ的Th1型和特定的产生Th2型细胞因子的CD4 +辅助性T细胞，这很可能是诱导p55特异性全身IgG和黏膜IgA反应的原因。
