Kawabata S, Miller C J, Lehner T, Fujihashi K, Kubota M, McGhee J R, Imaoka K, Hiroi T, Kiyono H
Department of Oral Biology, Immunobiology Vaccine Center, University of Alabama at Birmingham, USA.
J Infect Dis. 1998 Jan;177(1):26-33. doi: 10.1086/513811.
To determine if there is an association between the isotype of simian immunodeficiency virus (SIV)-specific B cell responses and the profile of Th1 and Th2 cytokine expression, rhesus macaques were immunized with SIV antigens via the iliac lymph nodes, using a targeted lymph node (TLN) immunization procedure. When CD4+ T cells purified from antigen-stimulated peripheral blood mononuclear cells were analyzed, the levels of Th2 cytokine production were gradually increased after the second and third immunizations. However, interferon-gamma production did not change. Analysis of SIV-specific B cell responses revealed that the main isotype was IgG after the second and third immunizations. In addition, a peak of SIV-specific IgA B cell responses was noted following the third immunization. These findings suggest that the induction of Th2 type responses in TLN-immunized rhesus macaques reflects the sequence of initial induction of SIV-specific IgG-producing cells followed by IgA-secreting cells.
为了确定猿猴免疫缺陷病毒（SIV）特异性B细胞应答的同种型与Th1和Th2细胞因子表达谱之间是否存在关联，采用靶向淋巴结（TLN）免疫程序，通过髂淋巴结用SIV抗原免疫恒河猴。当分析从抗原刺激的外周血单核细胞中纯化的CD4 + T细胞时，在第二次和第三次免疫后，Th2细胞因子产生水平逐渐升高。然而，γ干扰素的产生没有变化。对SIV特异性B细胞应答的分析显示，第二次和第三次免疫后主要同种型为IgG。此外，第三次免疫后观察到SIV特异性IgA B细胞应答出现峰值。这些发现表明，TLN免疫的恒河猴中Th2型应答的诱导反映了最初诱导产生SIV特异性IgG的细胞，随后是分泌IgA的细胞的顺序。
