Walde P, Marzetta B
Institut für Polymere, ETH-Zentrum, Universitätstrasse 6, CH-8092 Zürich, Switzerland.
Biotechnol Bioeng. 1998 Jan 20;57(2):216-9.
Liposomes were prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which contained the water soluble proteinase alpha-chymotrypsin. This liposome entrapped enzyme showed selectivity for externally added substrates in that only small substrates (benzoyl-l-Tyr-p-nitroanilide or acetyl-l-Phe-p-nitro-anilide)-for which the liposome bilayer was permeable-were transformed into products. Large substrates (succinyl-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide or casein) could not penetrate from the external aqueous phase into the liposomes, and were not hydrolyzed. This substrate selectivity is entirely based on the compartimentation and permeability properties of the liposome microreactor.
脂质体由1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）制备而成，其中包含水溶性蛋白酶α-胰凝乳蛋白酶。这种包封有酶的脂质体对外部添加的底物具有选择性，即只有小底物（苯甲酰-L-酪氨酸对硝基苯胺或乙酰-L-苯丙氨酸对硝基苯胺）——脂质体双层对其具有渗透性——能转化为产物。大底物（琥珀酰-L-丙氨酸-L-丙氨酸-L-脯氨酸-L-苯丙氨酸对硝基苯胺或酪蛋白）无法从外部水相渗透到脂质体中，因而不会被水解。这种底物选择性完全基于脂质体微反应器的分隔和渗透特性。
