Beaudoin D L, Bryers J D, Cunningham A B, Peretti S W
Department of Chemical, Bio and Materials Engineering, Arizona State University, Tempe, Arizona 85287, USA.
Biotechnol Bioeng. 1998 Feb 5;57(3):280-6.
A strain of Pseudomonas putida that harbors plasmids RK2 and pDLB101 was exposed to a pure culture biofilm of Bacillus azotoformans grown in a rotating annular reactor. Transfer of the RK2 mobilizable pDLB101 plasmid to B. azotoformans was monitored over a 4-day period. Experimental results demonstrated that the broad host range, RSF1010 derivative pDLB101 was transferred to and expressed by B. azotoformans. In the companion article to this work, the rate of plasmid transfer was quantified as a function of the limiting nutrient, succinate, and as a function of the mechanism of transfer. A biofilm process simulation program (AQUASIM) was modified to analyze resultant experimental data. Although the AQUASIM package was not designed to simulate or predict genetic events in biofilms, modification of the rate process dynamics allowed successful modeling of plasmid transfer. For the narrow range of substrate concentrations used in these experiments, nutrient level had only a slight effect on the rate and extent of plasmid transfer in biofilms. However, further simulations using AQUASIM revealed that under nutrient poor conditions, the number of transconjugants appearing in the biofilm was limited.
一株携带质粒RK2和pDLB101的恶臭假单胞菌暴露于在旋转环形反应器中生长的固氮芽孢杆菌纯培养生物膜中。在4天的时间内监测RK2可移动质粒pDLB101向固氮芽孢杆菌的转移。实验结果表明，广宿主范围的RSF1010衍生物pDLB101被转移至固氮芽孢杆菌并在其中表达。在这项工作的配套文章中，质粒转移速率被量化为限制性营养物质琥珀酸盐的函数以及转移机制的函数。对一个生物膜过程模拟程序（AQUASIM）进行了修改，以分析所得的实验数据。尽管AQUASIM软件包并非设计用于模拟或预测生物膜中的遗传事件，但对速率过程动力学的修改使得能够成功模拟质粒转移。对于这些实验中使用的较窄底物浓度范围，营养水平对生物膜中质粒转移速率和程度的影响很小。然而，使用AQUASIM进行的进一步模拟表明，在营养匮乏的条件下，生物膜中出现的接合子数量有限。
