Gu Z, Glatz B A, Glatz C E
Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011-2230, USA.
Biotechnol Bioeng. 1998 Feb 20;57(4):454-61.
Solvent selection for extractive fermentation for propionic acid was conducted with three systems: Alamine 304-1 (trilaurylamine) in 2-octanol, 1-dodecanol, and Witcohol 85 NF (oleyl alcohol). Among them, the solvent containing 2-octanol exhibited the highest partition coefficient in acid extraction, but it was also toxic to propionibacteria. The most solvent-resistant strain among five strains of the microorganism was selected. Solvent toxicity was eliminated via two strategies: entrapment of dissolved toxic solvent in the culture growth medium with vegetable oils such as corn, olive, or soybean oils; or replacement of the toxic 2-octanol with nontoxic Witcohol 85 NF. The complete recovery of acids from the Alamine 304-1/Witcohol 85 NF was also realized with vacuum distillation.
2 - 辛醇、1 - 十二醇和Witcohol 85 NF（油醇）中的阿拉明304 - 1（三(十二烷基)胺）。其中，含2 - 辛醇的溶剂在酸萃取中表现出最高的分配系数，但对丙酸杆菌也有毒性。从五种微生物菌株中筛选出了最耐溶剂的菌株。通过两种策略消除溶剂毒性：用玉米油、橄榄油或大豆油等植物油将溶解的有毒溶剂截留在培养基中；或者用无毒的Witcohol 85 NF替代有毒的2 - 辛醇。通过真空蒸馏也实现了从阿拉明304 - 1 / Witcohol 85 NF中完全回收酸。
