González-Peñas H, Lu-Chau T A, Moreira M T, Lema J M
IFP Energies Nouvelles, Rond-point de l'échangeur de Solaize BP 3, 69360, Solaize, France,
Appl Microbiol Biotechnol. 2014 Jul;98(13):5915-24. doi: 10.1007/s00253-014-5634-6. Epub 2014 Mar 28.
Solvent screening for in situ liquid extraction of products from acetone-butanol-ethanol (ABE) fermentation was carried out, taking into account biological parameters (biocompatibility, bioavailability, and product yield) and extraction performance (partition coefficient and selectivity) determined in real fermentation broth. On the basis of different solvent characteristics obtained from literature, 16 compounds from different chemical families were selected and experimentally evaluated for their extraction capabilities in a real ABE fermentation broth system. From these compounds, nine potential solvents were also tested for their biocompatibility towards Clostridium acetobutylicum. Moreover, bioavailability and differences in substrate consumption and total n-butanol production with respect to solvent-free fermentations were quantified for each biocompatible solvent. Product yield was enhanced in the presence of organic solvents having higher affinity for butanol and butyric acid. Applying this methodology, it was found that the Guerbet alcohol 2-butyl-1-octanol presented the best extracting characteristics (the highest partition coefficient (6.76) and the third highest selectivity (644)), the highest butanol yield (27.4 %), and maintained biocompatibility with C. acetobutylicum.
开展了从丙酮-丁醇-乙醇（ABE）发酵液中原位液液萃取产物的溶剂筛选工作，同时考虑了在实际发酵液中测定的生物学参数（生物相容性、生物可利用性和产物产率）以及萃取性能（分配系数和选择性）。基于从文献中获取的不同溶剂特性，从不同化学类别中选择了16种化合物，并在实际ABE发酵液体系中对其萃取能力进行了实验评估。从这些化合物中，还测试了9种潜在溶剂对丙酮丁醇梭菌的生物相容性。此外，对每种生物相容性溶剂的生物可利用性以及与无溶剂发酵相比底物消耗和总正丁醇产量的差异进行了量化。在对丁醇和丁酸具有更高亲和力的有机溶剂存在下，产物产率得到提高。应用该方法发现，格尔伯特醇2-丁基-1-辛醇表现出最佳的萃取特性（最高分配系数（6.76）和第三高的选择性（644））、最高的丁醇产率（27.4%），并与丙酮丁醇梭菌保持生物相容性。
