Chalmers J J, Zborowski M, Moore L, Mandal S, Fang B B, Sun L
Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210-1180, USA.
Biotechnol Bioeng. 1998 Jul 5;59(1):10-20.
A theoretical analysis was performed to determine the number of fractions a multidisperse, immunomagnetically labeled cell population can be separated into based on the surface marker (antigen) density. A number of assumptions were made in this analysis: that there is a proportionality between the number of surface markers on the cell surface and the number of immunomagnetic labels bound; that this surface marker density is independent of the cell diameter; and that there is only the presence of magnetic and drag forces acting on the cell. Due to the normal distribution of cell diameters, a "randomizing" effect enters into the analysis, and an analogy between the "theoretical plate" analysis of distillation, adsorption, and chromatography can be made. Using the experimentally determined, normal distribution of cell diameters for human lymphocytes and a breast cancer cell line, and fluorescent activated cell screening data of specific surface marker distributions, examples of theoretical plate calculations were made and discussed.
进行了一项理论分析，以确定基于表面标志物（抗原）密度，多分散的、免疫磁标记的细胞群体可被分离成的份数。该分析中做了若干假设：细胞表面的表面标志物数量与结合的免疫磁标记数量之间存在比例关系；该表面标志物密度与细胞直径无关；并且作用于细胞的仅存在磁力和曳力。由于细胞直径呈正态分布，“随机化”效应进入分析，并且可以在蒸馏、吸附和色谱的“理论塔板”分析之间进行类比。利用实验确定的人淋巴细胞和一种乳腺癌细胞系的细胞直径正态分布，以及特异性表面标志物分布的荧光激活细胞筛选数据，进行并讨论了理论塔板计算的实例。
