McCloskey Kara E, Moore Lee R, Hoyos Mauricio, Rodriguez Alex, Chalmers Jeffrey J, Zborowski Maciej
Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210.
Biotechnol Prog. 2003 May-Jun;19(3):899-907. doi: 10.1021/bp020285e.
Antibody binding capacity (ABC) is a term representing a cell's ability to bind antibodies, correlating with the number of specific cellular antigens expressed on that cell. ABC allows magnetically conjugated antibodies to bind to the targeted cells, imparting a magnetophoretic mobility on each targeted cell. This enables sorting based on differences in the cell magnetophoretic mobility and, potentially, a magnetic separation based on the differences in the cell ABC values. A cell's ABC value is a particularly important factor in continuous magnetic cell separation. This work investigates the relationship between ABC and magnetic cell separation efficiency by injection of a suspension of immunomagnetically labeled quantum simply cellular calibration microbeads of known ABC values into fluid flowing through a quadrupole magnetic sorter. The elution profiles of the outlet streams were evaluated using UV detectors. Optimal separation flow rate was shown to correlate with the ABC of these microbeads. Comparing experimental and theoretical results, the theory correctly predicted maximum separation flow rates but overestimated the separation fractional recoveries.
抗体结合能力（ABC）是一个表示细胞结合抗体能力的术语，与该细胞上表达的特定细胞抗原数量相关。ABC使磁偶联抗体能够结合到靶细胞上，赋予每个靶细胞磁泳迁移率。这使得能够基于细胞磁泳迁移率的差异进行分选，并有可能基于细胞ABC值的差异进行磁分离。细胞的ABC值是连续磁细胞分离中的一个特别重要的因素。这项工作通过将已知ABC值的免疫磁性标记量子简单细胞校准微珠悬浮液注入流经四极磁分选器的流体中，研究了ABC与磁细胞分离效率之间的关系。使用紫外探测器评估出口流的洗脱曲线。结果表明，最佳分离流速与这些微珠的ABC相关。比较实验结果和理论结果，该理论正确地预测了最大分离流速，但高估了分离分数回收率。
